2j5m
From Proteopedia
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| - | [[Image:2j5m.jpg|left|200px]]<br /><applet load="2j5m" size=" | + | [[Image:2j5m.jpg|left|200px]]<br /><applet load="2j5m" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j5m, resolution 1.75Å" /> | caption="2j5m, resolution 1.75Å" /> | ||
'''STRUCTURE OF CHLOROPEROXIDASE COMPOUND 0'''<br /> | '''STRUCTURE OF CHLOROPEROXIDASE COMPOUND 0'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J5M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with NAG, MAN, MN, ACT, HEM, PEO and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Act Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2J5M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MAN:'>MAN</scene>, <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=PEO:'>PEO</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: pyrrolidone carboxylic acid]] | [[Category: pyrrolidone carboxylic acid]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:41:49 2008'' |
Revision as of 08:41, 3 February 2008
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STRUCTURE OF CHLOROPEROXIDASE COMPOUND 0
Overview
We have determined the crystal structure of the chloroperoxidase (CPO), hydroperoxo reaction intermediate (CPO compound 0) at 1.75-A resolution., The intermediate was generated through controlled photoreduction of the, CPO oxygen complex during x-ray data collection, which was monitored by, recording of the crystal absorption spectra. Initially, the peroxo-anion, species was formed and then protonated to yield compound 0. Quantum, chemical calculations indicate that the peroxo-anion species is not stable, and collapses instantaneously to compound 0. Compound 0 is present in the, ferric low-spin doublet ground state and is characterized by a long O O, bond length of 1.5 A and a Fe O bond distance of 1.8 A, which is also, observed in the crystal structure.
About this Structure
2J5M is a Single protein structure of sequence from Leptoxyphium fumago with , , , , , and as ligands. Active as Chloride peroxidase, with EC number 1.11.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure and quantum chemical characterization of chloroperoxidase compound 0, a common reaction intermediate of diverse heme enzymes., Kuhnel K, Derat E, Terner J, Shaik S, Schlichting I, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):99-104. Epub 2006 Dec 26. PMID:17190816
Page seeded by OCA on Sun Feb 3 10:41:49 2008
Categories: Chloride peroxidase | Leptoxyphium fumago | Single protein | Derat, E. | Kuhnel, K. | Schlichting, I. | Shaik, S. | Terner, J. | ACT | EDO | HEM | MAN | MN | NAG | PEO | Chloride | Glycoprotein | Heme | Iron | Manganese | Metal-binding | Oxidoreductase | Peroxidase | Pyrrolidone carboxylic acid
