2j6h
From Proteopedia
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| - | [[Image:2j6h.gif|left|200px]]<br /><applet load="2j6h" size=" | + | [[Image:2j6h.gif|left|200px]]<br /><applet load="2j6h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j6h, resolution 2.35Å" /> | caption="2j6h, resolution 2.35Å" /> | ||
'''E. COLI GLUCOSAMINE-6-P SYNTHASE IN COMPLEX WITH GLUCOSE-6P AND 5-OXO-L-NORLEUCINE'''<br /> | '''E. COLI GLUCOSAMINE-6-P SYNTHASE IN COMPLEX WITH GLUCOSE-6P AND 5-OXO-L-NORLEUCINE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J6H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with G6Q and ONL as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 2BPJ. Active as [http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16] Known structural/functional Site: <scene name='pdbsite=AC1:G6q Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2J6H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=G6Q:'>G6Q</scene> and <scene name='pdbligand=ONL:'>ONL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 2BPJ. Active as [http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16] Known structural/functional Site: <scene name='pdbsite=AC1:G6q Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:43:07 2008'' |
Revision as of 12:43, 23 January 2008
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E. COLI GLUCOSAMINE-6-P SYNTHASE IN COMPLEX WITH GLUCOSE-6P AND 5-OXO-L-NORLEUCINE
Overview
Glucosamine-6P synthase catalyzes the synthesis of glucosamine-6P from, fructose-6P and glutamine and uses a channel to transfer ammonia from its, glutaminase to its synthase active site. X-ray structures of, glucosamine-6P synthase have been determined at 2.05 Angstroms resolution, in the presence of fructose-6P and at 2.35 Angstroms resolution in the, presence of fructose-6P and 6-diazo-5-oxo-L-norleucine, a glutamine, affinity analog that covalently modifies the N-terminal catalytic, cysteine, therefore mimicking the gamma-glutamyl-thioester intermediate, formed during hydrolysis of glutamine. The fixation of the glutamine, analog activates the enzyme through several major structural changes: 1), the closure of a loop to shield the glutaminase site accompanied by, significant domain hinging, 2) the activation of catalytic residues, involved in glutamine hydrolysis, i.e. the alpha-amino group of Cys-1 and, Asn-98 that is positioned to form the oxyanion hole, and 3) a 75 degrees, rotation of the Trp-74 indole group that opens the ammonia channel.
About this Structure
2J6H is a Single protein structure of sequence from Escherichia coli with and as ligands. This structure superseeds the now removed PDB entry 2BPJ. Active as Glutamine--fructose-6-phosphate transaminase (isomerizing), with EC number 2.6.1.16 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:16339762
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Categories: Escherichia coli | Glutamine--fructose-6-phosphate transaminase (isomerizing) | Single protein | Golinelli-Pimpaneau, B. | Mouilleron, S. | G6Q | ONL | 5-oxo-l-norleucine | Amidotransferase | Aminotransferase | Ammonia channeling | Direct protein sequencing | Glucosamine 6-phosphate synthase | Glucose 6-phosphate | Glutamine amidotransferase | N terminal nucleophile | Transferase
