2ja3
From Proteopedia
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| - | [[Image:2ja3.jpg|left|200px]]<br /><applet load="2ja3" size=" | + | [[Image:2ja3.jpg|left|200px]]<br /><applet load="2ja3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ja3, resolution 3.05Å" /> | caption="2ja3, resolution 3.05Å" /> | ||
'''CYTOPLASMIC DOMAIN OF THE HUMAN CHLORIDE TRANSPORTER CLC-5 IN COMPLEX WITH ADP'''<br /> | '''CYTOPLASMIC DOMAIN OF THE HUMAN CHLORIDE TRANSPORTER CLC-5 IN COMPLEX WITH ADP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Adp Binding Site For Chain D'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2JA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+D'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JA3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: voltage-gated channel]] | [[Category: voltage-gated channel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:43:20 2008'' |
Revision as of 08:43, 3 February 2008
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CYTOPLASMIC DOMAIN OF THE HUMAN CHLORIDE TRANSPORTER CLC-5 IN COMPLEX WITH ADP
Contents |
Overview
The ubiquitous CBS domains, which are found as part of cytoplasmic domains, in the ClC family of chloride channels and transporters, have previously, been identified as building blocks for regulatory nucleotide-binding, sites. Here we report the structures of the cytoplasmic domain of the, human transporter ClC-5 in complex with ATP and ADP. The nucleotides bind, to a specific site in the protein. As determined by equilibrium dialysis, the affinities for ATP, ADP and AMP are in the high micromolar range., Point mutations that interfere with nucleotide binding change the, transport behavior of a ClC-5 mutant expressed in Xenopus laevis oocytes., Our results establish the structural and energetic basis for the, interaction of ClC-5 with nucleotides and provide a framework for future, investigations.
Disease
Known diseases associated with this structure: Dent disease OMIM:[300008], Hypophosphatemic rickets OMIM:[300008], Nephrolithiasis, type I OMIM:[300008], Proteinuria, low molecular weight, with hypercalciuric nephrocalcinosis OMIM:[300008]
About this Structure
2JA3 is a Single protein structure of sequence from Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Nucleotide recognition by the cytoplasmic domain of the human chloride transporter ClC-5., Meyer S, Savaresi S, Forster IC, Dutzler R, Nat Struct Mol Biol. 2007 Jan;14(1):60-7. Epub 2006 Dec 31. PMID:17195847
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Categories: Homo sapiens | Single protein | Dutzler, R. | Forster, I.C. | Meyer, S. | Savaresi, S. | ADP | Cbs domain | Chloride | Chloride channel | Chloride transporter | Clc | Disease mutation | Domain | Ion transport | Ionic channel | Membrane | Transmembrane | Transport | Transport protein | Voltage-gated channel
