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1skf

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[[Category: serine peptidase]]
[[Category: serine peptidase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:53:37 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:04:12 2007''

Revision as of 13:59, 30 October 2007


1skf, resolution 2.00Å

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CRYSTAL STRUCTURE OF THE STREPTOMYCES K15 DD-TRANSPEPTIDASE

Overview

The serine DD-transpeptidase/penicillin-binding protein of Streptomyces, K15 catalyzes peptide bond formation in a way that mimics the, penicillin-sensitive peptide cross-linking reaction involved in bacterial, cell wall peptidoglycan assembly. The Streptomyces K15 enzyme is peculiar, in that it can be considered as an intermediate between classical, penicillin-binding proteins, for which benzylpenicillin is a very, efficient inactivator, and the resistant penicillin-binding proteins that, have a low penicillin affinity. With its moderate penicillin sensitivity, the Streptomyces K15 DD-transpeptidase would be helpful in the, understanding of the structure-activity relationship of this, penicillin-recognizing protein superfamily. The structure of the, Streptomyces K15 enzyme has been ... [(full description)]

About this Structure

1SKF is a [Single protein] structure of sequence from [Streptomyces sp.]. Active as [Serine-type D-Ala-D-Ala carboxypeptidase], with EC number [3.4.16.4]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].

Reference

The crystal structure of a penicilloyl-serine transferase of intermediate penicillin sensitivity. The DD-transpeptidase of streptomyces K15., Fonze E, Vermeire M, Nguyen-Disteche M, Brasseur R, Charlier P, J Biol Chem. 1999 Jul 30;274(31):21853-60. PMID:10419503

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