Ubiquitin

From Proteopedia

Revision as of 14:17, 20 September 2010

Template:STRUCTURE 1d3z

Ubiquitin (UBB) is found in almost all cells. It binds to proteins tagging them for destruction in the proteasome. UBB is activated by the UBB-activating enzymes E1, E2 and E3. UBB+1 is a frameshifted mutant of UBB observed in several diseases. A dimer of UBB (DiUBB) is formed by linkage of K48 to the C-terminus of a second UBB molecule. At least 4 UBB molecules are needed to tag a protein for the proteasome. The images at the left and at the right correspond to one representative Ubiquitin, i.e. the NMR structure of human Ubiquitin (1d3z).

3D Structures of Ubiquitin

Ubiquitin

2kn5, 2klg, 2jzz, 2pe9, 2pea, 1g6j, 1d3z – hUBB – NMR 2ojr, 2nr2, 2fcq, 1ubi, 1ubq - hUBB 2zcb, 2gbj, 2gbk, 2gbm, 2gbn, 2gbr, 2fcm, 2fcn, 2fcs, 1yiw, 1yj1, 1sif, 1ogw - hUBB (mutant) 1c3t, 1ud7 - hUBB (mutant) - NMR 1gjz – hUBB N-terminal – NMR 1e0q - hUBB N-terminal (mutant) – NMR 2k39 – UBB – Xenopus laevis – NMR 2jwz, 1zw7 – yUBB (mutant) – yeast 1cmx – yUBB (modified) 2zcc – bUBB - bovine

Ubiquitin+UBB-activating enzymes

3cmm – yUBB+E1 3k9p, 3a33 – hUBB+E2 2gmi - hUBB+E2+MMS2 2kjh, 1zgu – hUBB (mutant)+E2 3jvz, 3jw0 – hUBB+E2+E3

Ubiquitin+1 mutant

2kx0 – hUBB+1 – human – NMR 3k9o – hUBB+1+E2

Ubiquitin dimer

2xk5, 3nob, 2xew, 3h7p, 3h7s, 2w9n, 2jf5 – hDiUBB 3m3j, 1aar – bDiUBB 2bgf – hDiUBB – chemical relaxation

Ubiquitin tetramer

3alb, 3hm3, 2o6v, 1f9j, 1tbe – tetra-hUBB

Ubiquitin bound to protein

3ofi – hUBB+insulin protease 3n3k, 3ifw, 3kvf, 3kw5, 3irt, 3mtn, 3ihp – hUBB (mutant)+hUBB carboxyl-terminal esterase catalytic domain 3i3t, 2ibi, 2ayo, 1nbf - hUBB+hUBB carboxyl-terminal hydrolase catalytic domain 2hd5 - bUBB+hUBB carboxyl-terminal hydrolase catalytic domain 3ldz – bUBB+signal transducing adapter molecule 2khw – hUBB+immunoglobulin G-binding protein G 2kdi – yUBB/UIM fusion 1v80, 1v81 – bUBB/L40 ribosomal protein fusion - NMR 1yx5, 1yx6 - hUBB+26S proteasome non-ATPase regulatory subunit – NMR 2k6d – hUBB+SH3 domain-containing kinase-binding protein – NMR 2z59 – hUBB+mProtein Adhesion-regulating molecule 1 2jy6 – hUBB+hUbiquilin – NMR 3by4, 3c0r – hUBB+yUBB thioesterase 2jt4 – yUBB fragment+cytoskeleton assembly control protein 2hth – hUBB+vacuolar protein sorting protein 2dx5, 1p3q - bUBB+vacuolar protein sorting protein 1q0w - yUBB+vacuolar protein sorting protein 2g3q – yUBB+EDE1 UBA 2c7m, 2c7n, 2fid, 2fif – bUBB+RAB guanine nucleotide exchange factor 2d3g – bUBB+HRS-UIM 1wrd – bUBB+TOM1 GAT domain 1wr6, 1yd8 - bUBB+GGA3 GAT domain 1wr1 – yUBB+DSK2P UBA 1s1q – hUBB+tumor susceptibility gene 101 protein 1q5w – hUBB+NPL4 zinc-fingers 1uzx – bUBB+VPS23 UEV 1otr – yUBB+CUE2

Ubiquitin dimer bound to protein

3a9j, 3a9k, 2wwz, 2wx0, 2wx1 – hDiUBB+mitogen-activated protein kinase 3jsv, 2zvn, 2zvo – hDiUBB+NF-κ-b essential modulator 2kde, 2kdf – DiUBB+26S proteasome non-ATPase regulatory subunit – NMR 3a1q – mDiUBB+UBB interaction motif-containing protein – mouse 3dvg, 3dvn – hDiUBB+IGG1 fab light&heavy chains 2znv – mDiUBB+hAMSH-like protease

Ubiquitin + ions

3h1u – bUBB+Cd 3ehv, 3eec, 3efu – hUBB+ions