4pbg
From Proteopedia
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| - | [[Image:4pbg.gif|left|200px]]<br /><applet load="4pbg" size=" | + | [[Image:4pbg.gif|left|200px]]<br /><applet load="4pbg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="4pbg, resolution 2.5Å" /> | caption="4pbg, resolution 2.5Å" /> | ||
'''6-PHOSPHO-BETA-GALACTOSIDASE FORM-CST'''<br /> | '''6-PHOSPHO-BETA-GALACTOSIDASE FORM-CST'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 4PBG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with BGP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/6-phospho-beta-galactosidase 6-phospho-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.85 3.2.1.85] Known structural/functional Sites: <scene name='pdbsite=PHA:Phosphate Binding Site'>PHA</scene> and <scene name='pdbsite=PHB:Phosphate Binding Site'>PHB</scene>. Full crystallographic information is available from [http:// | + | 4PBG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with <scene name='pdbligand=BGP:'>BGP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/6-phospho-beta-galactosidase 6-phospho-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.85 3.2.1.85] Known structural/functional Sites: <scene name='pdbsite=PHA:Phosphate+Binding+Site'>PHA</scene> and <scene name='pdbsite=PHB:Phosphate+Binding+Site'>PHB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PBG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:53:03 2008'' |
Revision as of 08:53, 3 February 2008
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6-PHOSPHO-BETA-GALACTOSIDASE FORM-CST
Overview
The initial structural model of 6-phospho-beta-galactosidase from, Lactococcus lactis was refined to an R-factor of 16.4% (R[free] = 23.6%), to 2.3 A resolution (1 A = 0.1 nm), and the structures of three other, crystal forms were solved by molecular replacement. The four structural, models are essentially identical. The catalytic center of the enzyme is, approximately at the mass center of the molecule and can only be reached, through a 20 A long channel, which is observed with an "open" or "closed", entrance. The closed entrance is probably too small for the educt, lactose-6-phosphate to enter, but large enough for the first product, glucose to leave. Among the presented structures is a complex between an, almost inactive mutant and the second product galactose-6-phosphate, which, is exclusively bound at side-chains. A superposition (onto the native, enzyme) of galactose-6-phosphate as bound to the mutant suggests the, geometry of a postulated covalent intermediate. The binding mode of the, educt was modeled, starting from the bound galactose-6-phosphate. A, tightly fixed tryptophan is used as a chopping-board for splitting the, disaccharide, and several other aromatic residues in the active center, cavity are likely to participate in substrate transport/binding.
About this Structure
4PBG is a Single protein structure of sequence from Lactococcus lactis with as ligand. Active as 6-phospho-beta-galactosidase, with EC number 3.2.1.85 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Crystal structures and mechanism of 6-phospho-beta-galactosidase from Lactococcus lactis., Wiesmann C, Hengstenberg W, Schulz GE, J Mol Biol. 1997 Jun 27;269(5):851-60. PMID:9223646
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