This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3mp2
From Proteopedia
| Line 1: | Line 1: | ||
| - | {{Seed}} | ||
| - | [[Image:3mp2.png|left|200px]] | ||
| - | |||
| - | <!-- | ||
| - | The line below this paragraph, containing "STRUCTURE_3mp2", creates the "Structure Box" on the page. | ||
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet) | ||
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| - | or leave the SCENE parameter empty for the default display. | ||
| - | --> | ||
{{STRUCTURE_3mp2| PDB=3mp2 | SCENE= }} | {{STRUCTURE_3mp2| PDB=3mp2 | SCENE= }} | ||
| - | |||
===Crystal structure of transmissible gastroenteritis virus papain-like protease 1=== | ===Crystal structure of transmissible gastroenteritis virus papain-like protease 1=== | ||
| + | {{ABSTRACT_PUBMED_20668092}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/R1A_CVPPU R1A_CVPPU]] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). | |
| - | + | ||
| - | + | ||
| - | -- | + | |
| - | + | ||
==About this Structure== | ==About this Structure== | ||
| - | + | [[3mp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Transmissible_gastroenteritis_virus Transmissible gastroenteritis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MP2 OCA]. | |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:020668092</ref><references group="xtra"/><references/> |
| - | [[Category: | + | [[Category: Transmissible gastroenteritis virus]] |
[[Category: Manolaridis, I.]] | [[Category: Manolaridis, I.]] | ||
[[Category: Tucker, P A.]] | [[Category: Tucker, P A.]] | ||
| Line 32: | Line 19: | ||
[[Category: Papain-like protease]] | [[Category: Papain-like protease]] | ||
[[Category: Tgev]] | [[Category: Tgev]] | ||
| - | |||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:16:42 2010'' | ||
Revision as of 21:01, 17 April 2013
Contents |
Crystal structure of transmissible gastroenteritis virus papain-like protease 1
Template:ABSTRACT PUBMED 20668092
Function
[R1A_CVPPU] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).
About this Structure
3mp2 is a 1 chain structure with sequence from Transmissible gastroenteritis virus. Full crystallographic information is available from OCA.
Reference
- Wojdyla JA, Manolaridis I, van Kasteren PB, Kikkert M, Snijder EJ, Gorbalenya AE, Tucker PA. Papain-Like Protease 1 from Transmissible Gastroenteritis Virus: Crystal Structure and Enzymatic Activity toward Viral and Cellular Substrates. J Virol. 2010 Oct;84(19):10063-73. Epub 2010 Jul 28. PMID:20668092 doi:10.1128/JVI.00898-10
