3mp2
From Proteopedia
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{{STRUCTURE_3mp2| PDB=3mp2 | SCENE= }} | {{STRUCTURE_3mp2| PDB=3mp2 | SCENE= }} | ||
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===Crystal structure of transmissible gastroenteritis virus papain-like protease 1=== | ===Crystal structure of transmissible gastroenteritis virus papain-like protease 1=== | ||
| + | {{ABSTRACT_PUBMED_20668092}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/R1A_CVPPU R1A_CVPPU]] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). | |
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==About this Structure== | ==About this Structure== | ||
| - | + | [[3mp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Transmissible_gastroenteritis_virus Transmissible gastroenteritis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MP2 OCA]. | |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:020668092</ref><references group="xtra"/><references/> |
| - | [[Category: | + | [[Category: Transmissible gastroenteritis virus]] |
[[Category: Manolaridis, I.]] | [[Category: Manolaridis, I.]] | ||
[[Category: Tucker, P A.]] | [[Category: Tucker, P A.]] | ||
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[[Category: Papain-like protease]] | [[Category: Papain-like protease]] | ||
[[Category: Tgev]] | [[Category: Tgev]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:16:42 2010'' | ||
Revision as of 21:01, 17 April 2013
Contents |
Crystal structure of transmissible gastroenteritis virus papain-like protease 1
Template:ABSTRACT PUBMED 20668092
Function
[R1A_CVPPU] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).
About this Structure
3mp2 is a 1 chain structure with sequence from Transmissible gastroenteritis virus. Full crystallographic information is available from OCA.
Reference
- Wojdyla JA, Manolaridis I, van Kasteren PB, Kikkert M, Snijder EJ, Gorbalenya AE, Tucker PA. Papain-Like Protease 1 from Transmissible Gastroenteritis Virus: Crystal Structure and Enzymatic Activity toward Viral and Cellular Substrates. J Virol. 2010 Oct;84(19):10063-73. Epub 2010 Jul 28. PMID:20668092 doi:10.1128/JVI.00898-10
