2jmh
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Blo t 5 is the major allergen from Blomia tropicalis mites and shows | + | Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Roles of | + | Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18184590 18184590] |
[[Category: Blomia tropicalis]] | [[Category: Blomia tropicalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Huang, T.]] | [[Category: Huang, T.]] | ||
[[Category: Kuo, I.]] | [[Category: Kuo, I.]] | ||
| - | [[Category: Naik, M | + | [[Category: Naik, M T.]] |
[[Category: allergen]] | [[Category: allergen]] | ||
[[Category: blo t 5]] | [[Category: blo t 5]] | ||
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[[Category: group 5]] | [[Category: group 5]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:04:03 2008'' |
Revision as of 16:04, 21 February 2008
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NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis
Overview
Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases.
About this Structure
2JMH is a Single protein structure of sequence from Blomia tropicalis. Full crystallographic information is available from OCA.
Reference
Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:18184590
Page seeded by OCA on Thu Feb 21 18:04:03 2008
Categories: Blomia tropicalis | Single protein | Chang, C. | Chua, K. | Huang, T. | Kuo, I. | Naik, M T. | Allergen | Blo t 5 | Dust mites | Group 5
