2v7g

The analysis of natural contact interfaces between protein subunits and, between proteins has disclosed some general rules governing their, association. We have applied these rules to produce a number of novel, assemblies, demonstrating that a given protein can be engineered to form, contacts at various points of its surface. Symmetry plays an important, role because it defines the multiplicity of a designed contact and, therefore the number of required mutations. Some of the proteins needed, only a single side-chain alteration in order to associate to a, higher-order complex. The mobility of the buried side chains has to be, taken into account. Four assemblies have been structurally elucidated., Comparisons between the designed contacts and the results will provide, useful guidelines for the development of future architectures.

2V7G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=NAD:'>NAD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urocanate_hydratase Urocanate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.49 4.2.1.49] Known structural/functional Sites: <scene name='pdbsite=AC1:Nad Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Nad Binding Site For Chain B'>AC2</scene>, <scene name='pdbsite=AC3:Nad Binding Site For Chain C'>AC3</scene>, <scene name='pdbsite=AC4:Nad Binding Site For Chain D'>AC4</scene>, <scene name='pdbsite=AC5:Act Binding Site For Chain C'>AC5</scene>, <scene name='pdbsite=AC6:Act Binding Site For Chain B'>AC6</scene>, <scene name='pdbsite=AC7:Act Binding Site For Chain B'>AC7</scene>, <scene name='pdbsite=AC8:Act Binding Site For Chain A'>AC8</scene>, <scene name='pdbsite=AC9:Act Binding Site For Chain B'>AC9</scene>, <scene name='pdbsite=BC1:Act Binding Site For Chain A'>BC1</scene>, <scene name='pdbsite=BC2:Act Binding Site For Chain C'>BC2</scene>, <scene name='pdbsite=BC3:Gol Binding Site For Chain B'>BC3</scene>, <scene name='pdbsite=BC4:Gol Binding Site For Chain D'>BC4</scene>, <scene name='pdbsite=BC5:Gol Binding Site For Chain B'>BC5</scene>, <scene name='pdbsite=BC6:Gol Binding Site For Chain D'>BC6</scene>, <scene name='pdbsite=BC7:Gol Binding Site For Chain A'>BC7</scene>, <scene name='pdbsite=BC8:Gol Binding Site For Chain D'>BC8</scene>, <scene name='pdbsite=BC9:Gol Binding Site For Chain B'>BC9</scene>, <scene name='pdbsite=CC1:Gol Binding Site For Chain A'>CC1</scene> and <scene name='pdbsite=CC2:Gol Binding Site For Chain D'>CC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V7G OCA].

[[Category: Schulz, G.E.]]

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