3b31
From Proteopedia
(New page: 200px<br /><applet load="3b31" size="350" color="white" frame="true" align="right" spinBox="true" caption="3b31, resolution 2.40Å" /> '''Crystal structure of...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Internal ribosome entry site (IRES) RNAs initiate protein synthesis in | + | Internal ribosome entry site (IRES) RNAs initiate protein synthesis in eukaryotic cells by a noncanonical cap-independent mechanism. IRESes are critical for many pathogenic viruses, but efforts to understand their function are complicated by the diversity of IRES sequences as well as by limited high-resolution structural information. The intergenic region (IGR) IRESes of the Dicistroviridae viruses are powerful model systems to begin to understand IRES function. Here we present the crystal structure of a Dicistroviridae IGR IRES domain that interacts with the ribosome's decoding groove. We find that this RNA domain precisely mimics the transfer RNA anticodon-messenger RNA codon interaction, and its modeled orientation on the ribosome helps explain translocation without peptide bond formation. When combined with a previous structure, this work completes the first high-resolution description of an IRES RNA and provides insight into how RNAs can manipulate complex biological machines. |
==About this Structure== | ==About this Structure== | ||
| Line 12: | Line 12: | ||
tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18157151 18157151] | tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18157151 18157151] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Costantino, D | + | [[Category: Costantino, D A.]] |
| - | [[Category: Kieft, J | + | [[Category: Kieft, J S.]] |
| - | [[Category: Pfingsten, J | + | [[Category: Pfingsten, J S.]] |
| - | [[Category: Rambo, R | + | [[Category: Rambo, R P.]] |
[[Category: IRI]] | [[Category: IRI]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
| Line 25: | Line 25: | ||
[[Category: trna mimicry]] | [[Category: trna mimicry]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:57 2008'' |
Revision as of 17:02, 21 February 2008
|
Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA
Overview
Internal ribosome entry site (IRES) RNAs initiate protein synthesis in eukaryotic cells by a noncanonical cap-independent mechanism. IRESes are critical for many pathogenic viruses, but efforts to understand their function are complicated by the diversity of IRES sequences as well as by limited high-resolution structural information. The intergenic region (IGR) IRESes of the Dicistroviridae viruses are powerful model systems to begin to understand IRES function. Here we present the crystal structure of a Dicistroviridae IGR IRES domain that interacts with the ribosome's decoding groove. We find that this RNA domain precisely mimics the transfer RNA anticodon-messenger RNA codon interaction, and its modeled orientation on the ribosome helps explain translocation without peptide bond formation. When combined with a previous structure, this work completes the first high-resolution description of an IRES RNA and provides insight into how RNAs can manipulate complex biological machines.
About this Structure
3B31 is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:18157151
Page seeded by OCA on Thu Feb 21 19:02:57 2008
