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Gyrase
From Proteopedia
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[[1ajb]] - EcGyrA N-terminal+novobiocin<br /> | [[1ajb]] - EcGyrA N-terminal+novobiocin<br /> | ||
[[1zi0]], [[1ab4]] - EcGyrA C-terminal<br /> | [[1zi0]], [[1ab4]] - EcGyrA C-terminal<br /> | ||
| + | [[3ku8]] – EcGyrA fragment+CcdB<br /> | ||
[[1x75]] – EcGyrA14+CcdB<br /> | [[1x75]] – EcGyrA14+CcdB<br /> | ||
| - | [[3ilw]] - MtGyrA N-terminal – ''Mycobacterium tuberculosis''<br /> | + | [[3kua]] - GyrA fragment+CcdB – ''Vibrio fischeri''<br /> |
| + | [[3ilw]], [[3ifz]] - MtGyrA N-terminal – ''Mycobacterium tuberculosis''<br /> | ||
[[1suu]] - GyrA C-terminal – ''Borrelia burgdorferi''<br /> | [[1suu]] - GyrA C-terminal – ''Borrelia burgdorferi''<br /> | ||
| + | [[3no0]] - GyrA C-terminal – ''Aquifex aeolicus'' | ||
===Gyrase Subunit B=== | ===Gyrase Subunit B=== | ||
[[3g75]], [[3g7b]], [[3g7e]] – GyrB+thiazole inhibitor – ''Staphylococcus aureus''<br /> | [[3g75]], [[3g7b]], [[3g7e]] – GyrB+thiazole inhibitor – ''Staphylococcus aureus''<br /> | ||
| - | [[ | + | [[2zjt]], [[3ig0]], [[3m4i]] - MtGyrB C-terminal<br /> |
[[3cwv]] – GyrB truncated – ''Myxococcus xanthus''<br /> | [[3cwv]] – GyrB truncated – ''Myxococcus xanthus''<br /> | ||
[[1kzn]], [[1ei1]] - EcGyrB N-terminal+clorobiocin<br /> | [[1kzn]], [[1ei1]] - EcGyrB N-terminal+clorobiocin<br /> | ||
| + | [[1aj6]] - EcGyrB N-terminal+novobiocin<br /> | ||
[[1kij]] – GyrB domain+novobiocin – ''Thermus thermophilus''<br /> | [[1kij]] – GyrB domain+novobiocin – ''Thermus thermophilus''<br /> | ||
| + | |||
| + | ===Gyrase Subunit A+Subunit B=== | ||
| + | |||
| + | [[2xco]], [[2xcq]] - SaGyrB C-terminal-SaGyrA N-terminal fusion<br /> | ||
| + | [[2xcr]], [[2xcs]] - SaGyrB C-terminal-SaGyrA N-terminal fusion (mutant)+DNA<br /> | ||
| + | [[2xct]] - SaGyrB C-terminal-SaGyrA N-terminal fusion (mutant) +DNA+ ciprofloxacin<br /> | ||
| + | [[3nuh]] – EcGyrA+EcGyrB | ||
===Reverse Gyrase=== | ===Reverse Gyrase=== | ||
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[[1gku]] – AfTop-RG – ''Archaeoglobus fulgidus''<br /> | [[1gku]] – AfTop-RG – ''Archaeoglobus fulgidus''<br /> | ||
[[1gl9]] - AfTop-RG+ADPNP<br /> | [[1gl9]] - AfTop-RG+ADPNP<br /> | ||
| + | [[3oiy]] – Top-RG helicase domain – ''Thermotoga maritima'' | ||
==Additional Resources== | ==Additional Resources== | ||
Revision as of 10:55, 21 December 2010
|
Gyrase (Gyr) is a type of topoisomerase II in prokaryotes which unwinds double stranded DNA. The DNA Gyr cutting allows the formation of a negative DNA supercoil which enables replication of DNA. Gyr consists of 2 subunits: GyrA and GyrB. Reverse gyrase (Top-RG) is a type of topoisomerase I which catalyses the formation of positive DNA supercoil. [1]
Contents |
3D Structure of Gyrase
Gyrase Subunit A
3l6v – GyrA C-terminal – Xanthomonas campestris
2wl2 – EcGyrA N-terminal+simocylinone – Escherichia coli
1ajb - EcGyrA N-terminal+novobiocin
1zi0, 1ab4 - EcGyrA C-terminal
3ku8 – EcGyrA fragment+CcdB
1x75 – EcGyrA14+CcdB
3kua - GyrA fragment+CcdB – Vibrio fischeri
3ilw, 3ifz - MtGyrA N-terminal – Mycobacterium tuberculosis
1suu - GyrA C-terminal – Borrelia burgdorferi
3no0 - GyrA C-terminal – Aquifex aeolicus
Gyrase Subunit B
3g75, 3g7b, 3g7e – GyrB+thiazole inhibitor – Staphylococcus aureus
2zjt, 3ig0, 3m4i - MtGyrB C-terminal
3cwv – GyrB truncated – Myxococcus xanthus
1kzn, 1ei1 - EcGyrB N-terminal+clorobiocin
1aj6 - EcGyrB N-terminal+novobiocin
1kij – GyrB domain+novobiocin – Thermus thermophilus
Gyrase Subunit A+Subunit B
2xco, 2xcq - SaGyrB C-terminal-SaGyrA N-terminal fusion
2xcr, 2xcs - SaGyrB C-terminal-SaGyrA N-terminal fusion (mutant)+DNA
2xct - SaGyrB C-terminal-SaGyrA N-terminal fusion (mutant) +DNA+ ciprofloxacin
3nuh – EcGyrA+EcGyrB
Reverse Gyrase
1gku – AfTop-RG – Archaeoglobus fulgidus
1gl9 - AfTop-RG+ADPNP
3oiy – Top-RG helicase domain – Thermotoga maritima
Additional Resources
For additional information, see: Bacterial Infections
References
- ↑ Gore J, Bryant Z, Stone MD, Nollmann M, Cozzarelli NR, Bustamante C. Mechanochemical analysis of DNA gyrase using rotor bead tracking. Nature. 2006 Jan 5;439(7072):100-4. PMID:16397501 doi:10.1038/nature04319
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Michal Harel, Alexander Berchansky, David Canner, Joel L. Sussman
