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1e7q

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Revision as of 13:02, 30 October 2007


1e7q, resolution 1.6Å

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GDP 4-KETO-6-DEOXY-D-MANNOSE EPIMERASE REDUCTASE S107A

Overview

GDP-4-keto-6-deoxy-d-mannose epimerase/reductase is a bifunctional enzyme, responsible for the last step in the biosynthesis of GDP-l-fucose, the, substrate of fucosyl transferases. Several cell-surface antigens, including the leukocyte Lewis system and cell-surface antigens in, pathogenic bacteria, depend on the availability of GDP-l-fucose for their, expression. Therefore, the enzyme is a potential target for therapy in, pathological states depending on selectin-mediated cell-to-cell, interactions. Previous crystallographic investigations have shown that, GDP-4-keto-6-deoxy-d-mannose epimerase/reductase belongs to the, short-chain dehydrogenase/reductase protein homology family. The enzyme, active-site region is at the interface of an N-terminal NADPH-binding, domain and a C-terminal ... [(full description)]

About this Structure

1E7Q is a [Single protein] structure of sequence from [Escherichia coli] with SO4, NAP, UVW and TRS as [ligands]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [OCA].

Reference

Probing the catalytic mechanism of GDP-4-keto-6-deoxy-d-mannose Epimerase/Reductase by kinetic and crystallographic characterization of site-specific mutants., Rosano C, Bisso A, Izzo G, Tonetti M, Sturla L, De Flora A, Bolognesi M, J Mol Biol. 2000 Oct 13;303(1):77-91. PMID:11021971

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