3p0b
From Proteopedia
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{{STRUCTURE_3p0b| PDB=3p0b | SCENE= }} | {{STRUCTURE_3p0b| PDB=3p0b | SCENE= }} | ||
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===Thermus thermophilus family GH57 branching enzyme: crystal structure, mechanism of action and products formed=== | ===Thermus thermophilus family GH57 branching enzyme: crystal structure, mechanism of action and products formed=== | ||
| + | {{ABSTRACT_PUBMED_021097495}} | ||
| + | ==Function== | ||
| + | [[http://www.uniprot.org/uniprot/P84162_THETH P84162_THETH]] Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 3 to 13, with two local maxima at DP 7 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Is involved in glycogen biosynthesis. Shows a secondary activity, i.e. the hydrolysis of the substrate, being 4% of the total activity. Can use amylose as substrate but not alpha-1,4-linked oligosaccharides of 2-7 glucose residues, beta-cyclodextrin, 6-O-glucosyl-beta-cyclodextrin and 6-O-maltosyl-beta-cyclodextrin. Is not able to branch amylopectin further, it only hydrolyzes amylopectin. Thus, displays preference for linear and long substrates (amylose) over branched structures (amylopectin). | ||
==About this Structure== | ==About this Structure== | ||
| - | + | [[3p0b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P0B OCA]. | |
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Dijkstra, B W.]] | [[Category: Dijkstra, B W.]] | ||
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[[Category: Glycoside hydrolase gh57]] | [[Category: Glycoside hydrolase gh57]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 27 11:29:41 2010'' | ||
Revision as of 08:29, 2 May 2013
Thermus thermophilus family GH57 branching enzyme: crystal structure, mechanism of action and products formed
Template:ABSTRACT PUBMED 021097495
Function
[P84162_THETH] Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 3 to 13, with two local maxima at DP 7 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Is involved in glycogen biosynthesis. Shows a secondary activity, i.e. the hydrolysis of the substrate, being 4% of the total activity. Can use amylose as substrate but not alpha-1,4-linked oligosaccharides of 2-7 glucose residues, beta-cyclodextrin, 6-O-glucosyl-beta-cyclodextrin and 6-O-maltosyl-beta-cyclodextrin. Is not able to branch amylopectin further, it only hydrolyzes amylopectin. Thus, displays preference for linear and long substrates (amylose) over branched structures (amylopectin).
About this Structure
3p0b is a 1 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
