3bdq
From Proteopedia
(New page: 200px<br /><applet load="3bdq" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bdq, resolution 2.000Å" /> '''Room Tempreture Cry...) |
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==Overview== | ==Overview== | ||
| - | Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier | + | Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-2-like2 (AeSCP-2L2) are members of the SCP-2 protein family with similar expression profiles in the mosquito life cycle. In an effort to understand how lipids can be transported by different SCP-2 proteins, the three-dimensional crystal structure of AeSCP-2L2 was solved at 1.7 A resolution. AeSCP-2L2 forms a dimer and binds three fatty acids, one of which resides in a position within the internal cavity at a right angle to the others. This first report of ligand-bound dimerized protein in the SCP-2 protein family indicates that the family has a much more divergent mode of interaction with ligands than previously reported. The potential function of AeSCP-2L2 was investigated via in vivo incorporation of [(3)H]cholesterol and [(3)H]palmitic acid. Overexpression of AeSCP-2L2 in mosquito cells leads to an increased uptake of free fatty acid, whereas knockdown of AeSCP-2L2 in adult females decreases the accumulation of free fatty acid in the fat body from a blood meal. In contrast, overexpression or knockdown of AeSCP-2L2 has no effect on cholesterol uptake. Our results suggest that the main function of AeSCP-2L2 is as a general intracellular fatty acid carrier, as opposed to having a dedicated role in cholesterol transport. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | + | Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18084051 18084051] | |
[[Category: Aedes aegypti]] | [[Category: Aedes aegypti]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Dyer, D | + | [[Category: Dyer, D H.]] |
| - | [[Category: Forest, K | + | [[Category: Forest, K T.]] |
[[Category: Lan, Q.]] | [[Category: Lan, Q.]] | ||
[[Category: PLM]] | [[Category: PLM]] | ||
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[[Category: sterol carrier protein]] | [[Category: sterol carrier protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:05:01 2008'' |
Revision as of 17:05, 21 February 2008
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Room Tempreture Crystal Structure of Sterol Carrier Protein-2 Like-2
Overview
Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-2-like2 (AeSCP-2L2) are members of the SCP-2 protein family with similar expression profiles in the mosquito life cycle. In an effort to understand how lipids can be transported by different SCP-2 proteins, the three-dimensional crystal structure of AeSCP-2L2 was solved at 1.7 A resolution. AeSCP-2L2 forms a dimer and binds three fatty acids, one of which resides in a position within the internal cavity at a right angle to the others. This first report of ligand-bound dimerized protein in the SCP-2 protein family indicates that the family has a much more divergent mode of interaction with ligands than previously reported. The potential function of AeSCP-2L2 was investigated via in vivo incorporation of [(3)H]cholesterol and [(3)H]palmitic acid. Overexpression of AeSCP-2L2 in mosquito cells leads to an increased uptake of free fatty acid, whereas knockdown of AeSCP-2L2 in adult females decreases the accumulation of free fatty acid in the fat body from a blood meal. In contrast, overexpression or knockdown of AeSCP-2L2 has no effect on cholesterol uptake. Our results suggest that the main function of AeSCP-2L2 is as a general intracellular fatty acid carrier, as opposed to having a dedicated role in cholesterol transport.
About this Structure
3BDQ is a Single protein structure of sequence from Aedes aegypti with as ligand. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:18084051
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