2p7v
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The Escherichia coli Rsd protein binds tightly and specifically to the RNA | + | The Escherichia coli Rsd protein binds tightly and specifically to the RNA polymerase (RNAP) sigma(70) factor. Rsd plays a role in alternative sigma factor-dependent transcription by biasing the competition between sigma(70) and alternative sigma factors for the available core RNAP. Here, we determined the 2.6 A-resolution X-ray crystal structure of Rsd bound to sigma(70) domain 4 (sigma(70)(4)), the primary determinant for Rsd binding within sigma(70). The structure reveals that Rsd binding interferes with the two primary functions of sigma(70)(4), core RNAP binding and promoter -35 element binding. Interestingly, the most highly conserved Rsd residues form a network of interactions through the middle of the Rsd structure that connect the sigma(70)(4)-binding surface with three cavities exposed on distant surfaces of Rsd, suggesting functional coupling between sigma(70)(4) binding and other binding surfaces of Rsd, either for other proteins or for as yet unknown small molecule effectors. These results provide a structural basis for understanding the role of Rsd, as well as its ortholog, AlgQ, a positive regulator of Pseudomonas aeruginosa virulence, in transcription regulation. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Crystal | + | Crystal structure of the Escherichia coli regulator of sigma70, Rsd, in complex with sigma70 domain 4., Patikoglou GA, Westblade LF, Campbell EA, Lamour V, Lane WJ, Darst SA, J Mol Biol. 2007 Sep 21;372(3):649-59. Epub 2007 Jul 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17681541 17681541] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Campbell, E | + | [[Category: Campbell, E A.]] |
| - | [[Category: Darst, S | + | [[Category: Darst, S A.]] |
[[Category: Lamour, V.]] | [[Category: Lamour, V.]] | ||
| - | [[Category: Lane, W | + | [[Category: Lane, W J.]] |
| - | [[Category: Patikoglou, G | + | [[Category: Patikoglou, G A.]] |
| - | [[Category: Westblade, L | + | [[Category: Westblade, L F.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: e. coli rsd-sigma 70 complex]] | [[Category: e. coli rsd-sigma 70 complex]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:26:53 2008'' |
Revision as of 16:26, 21 February 2008
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Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4
Overview
The Escherichia coli Rsd protein binds tightly and specifically to the RNA polymerase (RNAP) sigma(70) factor. Rsd plays a role in alternative sigma factor-dependent transcription by biasing the competition between sigma(70) and alternative sigma factors for the available core RNAP. Here, we determined the 2.6 A-resolution X-ray crystal structure of Rsd bound to sigma(70) domain 4 (sigma(70)(4)), the primary determinant for Rsd binding within sigma(70). The structure reveals that Rsd binding interferes with the two primary functions of sigma(70)(4), core RNAP binding and promoter -35 element binding. Interestingly, the most highly conserved Rsd residues form a network of interactions through the middle of the Rsd structure that connect the sigma(70)(4)-binding surface with three cavities exposed on distant surfaces of Rsd, suggesting functional coupling between sigma(70)(4) binding and other binding surfaces of Rsd, either for other proteins or for as yet unknown small molecule effectors. These results provide a structural basis for understanding the role of Rsd, as well as its ortholog, AlgQ, a positive regulator of Pseudomonas aeruginosa virulence, in transcription regulation.
About this Structure
2P7V is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Escherichia coli regulator of sigma70, Rsd, in complex with sigma70 domain 4., Patikoglou GA, Westblade LF, Campbell EA, Lamour V, Lane WJ, Darst SA, J Mol Biol. 2007 Sep 21;372(3):649-59. Epub 2007 Jul 3. PMID:17681541
Page seeded by OCA on Thu Feb 21 18:26:53 2008
Categories: Escherichia coli | Protein complex | Campbell, E A. | Darst, S A. | Lamour, V. | Lane, W J. | Patikoglou, G A. | Westblade, L F. | MG | E. coli rsd-sigma 70 complex | Escherichia coli rsd-sigma 70 complex | Helix-turn-helix | Regulation | Regulator of sigma 70 | Rsd | Rsd-sigma 70 complex | Sigma 70 | Sigma 70 domain 4 | Sigma factor | Transcription
