2olp
From Proteopedia
(New page: 200px<br /><applet load="2olp" size="350" color="white" frame="true" align="right" spinBox="true" caption="2olp, resolution 1.932Å" /> '''Structure and ligan...) |
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==Overview== | ==Overview== | ||
| - | Haemoglobin II is one of three haemoglobins present in the cytoplasm of | + | Haemoglobin II is one of three haemoglobins present in the cytoplasm of the Lucina pectinata mollusc that inhabits the Caribbean coast. Using HBII purified from its natural source, crystallization screening was performed using the counter-diffusion method with capillaries of 0.2 mm inner diameter. Crystals of HbII suitable for data collection and structure determination were grown in the presence of agarose at 0.1%(w/v) in order to improve their quality. The crystals belong to the tetragonal space group P4(2)2(1)2, with unit-cell parameters a = b = 73.92, c = 152.35 A, and diffracted X-rays to a resolution of better than 2.0 A. The asymmetric unit is a homodimer with a corresponding Matthews coefficient (VM) of 3.15 A3 Da(-1) and a solvent content of 61% by volume. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Camara-Artigas, A.]] | [[Category: Camara-Artigas, A.]] | ||
| - | [[Category: Garcia-Ruiz, J | + | [[Category: Garcia-Ruiz, J M.]] |
| - | [[Category: Gavira, J | + | [[Category: Gavira, J A.]] |
| - | [[Category: Jesus, W | + | [[Category: Jesus, W de.]] |
[[Category: Lopez-Garriga, J.]] | [[Category: Lopez-Garriga, J.]] | ||
[[Category: HEM]] | [[Category: HEM]] | ||
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[[Category: oxygen transport]] | [[Category: oxygen transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:56 2008'' |
Revision as of 16:19, 21 February 2008
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Structure and ligand selection of hemoglobin II from Lucina pectinata
Overview
Haemoglobin II is one of three haemoglobins present in the cytoplasm of the Lucina pectinata mollusc that inhabits the Caribbean coast. Using HBII purified from its natural source, crystallization screening was performed using the counter-diffusion method with capillaries of 0.2 mm inner diameter. Crystals of HbII suitable for data collection and structure determination were grown in the presence of agarose at 0.1%(w/v) in order to improve their quality. The crystals belong to the tetragonal space group P4(2)2(1)2, with unit-cell parameters a = b = 73.92, c = 152.35 A, and diffracted X-rays to a resolution of better than 2.0 A. The asymmetric unit is a homodimer with a corresponding Matthews coefficient (VM) of 3.15 A3 Da(-1) and a solvent content of 61% by volume.
About this Structure
2OLP is a Single protein structure of sequence from Lucina pectinata with , and as ligands. Full crystallographic information is available from OCA.
Reference
Capillary crystallization and molecular-replacement solution of haemoglobin II from the clam Lucina pectinata., Gavira JA, de Jesus W, Camara-Artigas A, Lopez-Garriga J, Garcia-Ruiz JM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):196-9. Epub 2006 Feb 10. PMID:16511300
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