2pk9
From Proteopedia
(New page: 200px<br /><applet load="2pk9" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pk9, resolution 2.906Å" /> '''Structure of the Ph...) |
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==Overview== | ==Overview== | ||
| - | The ability to sense and respond appropriately to environmental changes is | + | The ability to sense and respond appropriately to environmental changes is a primary requirement of all living organisms. In response to phosphate limitation, Saccharomyces cerevisiae induces transcription of a set of genes involved in the regulation of phosphate acquisition from the ambient environment. A signal transduction pathway (the PHO pathway) mediates this response, with Pho85-Pho80 playing a vital role. Here we report the X-ray structure of Pho85-Pho80, a prototypic structure of a CDK-cyclin complex functioning in transcriptional regulation in response to environmental changes. The structure revealed a specific salt link between a Pho85 arginine and a Pho80 aspartate that makes phosphorylation of the Pho85 activation loop dispensable and that maintains a Pho80 loop conformation for possible substrate recognition. It further showed two sites on the Pho80 cyclin for high-affinity binding of the transcription factor substrate (Pho4) and the CDK inhibitor (Pho81) that are markedly distant to each other and the active site. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Structure of the Pho85-Pho80 CDK- | + | Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18042456 18042456] |
[[Category: Cyclin-dependent kinase]] | [[Category: Cyclin-dependent kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Connell, I | + | [[Category: Connell, I Ferrin-O.]] |
[[Category: Huang, K.]] | [[Category: Huang, K.]] | ||
| - | [[Category: Leonard, G | + | [[Category: Leonard, G A.]] |
| - | [[Category: Quiocho, F | + | [[Category: Quiocho, F A.]] |
| - | [[Category: Shea, E | + | [[Category: Shea, E K.O.]] |
[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
[[Category: MES]] | [[Category: MES]] | ||
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[[Category: transferase/cell cycle complex]] | [[Category: transferase/cell cycle complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:30:19 2008'' |
Revision as of 16:30, 21 February 2008
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Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway
Overview
The ability to sense and respond appropriately to environmental changes is a primary requirement of all living organisms. In response to phosphate limitation, Saccharomyces cerevisiae induces transcription of a set of genes involved in the regulation of phosphate acquisition from the ambient environment. A signal transduction pathway (the PHO pathway) mediates this response, with Pho85-Pho80 playing a vital role. Here we report the X-ray structure of Pho85-Pho80, a prototypic structure of a CDK-cyclin complex functioning in transcriptional regulation in response to environmental changes. The structure revealed a specific salt link between a Pho85 arginine and a Pho80 aspartate that makes phosphorylation of the Pho85 activation loop dispensable and that maintains a Pho80 loop conformation for possible substrate recognition. It further showed two sites on the Pho80 cyclin for high-affinity binding of the transcription factor substrate (Pho4) and the CDK inhibitor (Pho81) that are markedly distant to each other and the active site.
About this Structure
2PK9 is a Protein complex structure of sequences from Saccharomyces cerevisiae with as ligand. Active as Cyclin-dependent kinase, with EC number 2.7.11.22 Full crystallographic information is available from OCA.
Reference
Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:18042456
Page seeded by OCA on Thu Feb 21 18:30:19 2008
