2vas
From Proteopedia
(New page: 200px<br /><applet load="2vas" size="350" color="white" frame="true" align="right" spinBox="true" caption="2vas, resolution 2.40Å" /> '''MYOSIN VI (MD-INSERT...) |
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==Overview== | ==Overview== | ||
| - | Myosin VI has an unexpectedly large swing of its lever arm (powerstroke) | + | Myosin VI has an unexpectedly large swing of its lever arm (powerstroke) that optimizes its unique reverse direction movement. The basis for this is an unprecedented rearrangement of the subdomain to which the lever arm is attached, referred to as the converter. It is unclear at what point(s) in the myosin VI ATPase cycle rearrangements in the converter occur, and how this would effect lever arm position. We solved the structure of myosin VI with an ATP analogue (ADP.BeF3) bound in its nucleotide-binding pocket. The structure reveals that no rearrangement in the converter occur upon ATP binding. Based on previously solved myosin structures, our structure suggests that no reversal of the powerstroke occurs during detachment of myosin VI from actin. The structure also reveals novel features of the myosin VI motor that may be important in maintaining the converter conformation during detachment from actin, and other features that may promote rapid rearrangements in the structure following actin detachment that enable hydrolysis of ATP. |
==About this Structure== | ==About this Structure== | ||
| - | 2VAS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=BEF:'>BEF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Adp Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Bef Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Mg Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Ca Binding Site For Chain B'>AC4</scene>, <scene name='pdbsite=AC5:Ca Binding Site For Chain B'>AC5</scene>, <scene name='pdbsite=AC6:Ca Binding Site For Chain B'>AC6</scene> and <scene name='pdbsite=AC7:Ca Binding Site For Chain B'>AC7</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAS OCA]. | + | 2VAS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=BEF:'>BEF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Bef+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Ca+Binding+Site+For+Chain+B'>AC5</scene>, <scene name='pdbsite=AC6:Ca+Binding+Site+For+Chain+B'>AC6</scene> and <scene name='pdbsite=AC7:Ca+Binding+Site+For+Chain+B'>AC7</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAS OCA]. |
==Reference== | ==Reference== | ||
| - | The post-rigor structure of myosin VI and implications for the recovery stroke., Menetrey J, Llinas P, Cicolari J, Squires G, Liu X, Li A, Sweeney HL, Houdusse A, EMBO J. 2007 Nov 29 | + | The post-rigor structure of myosin VI and implications for the recovery stroke., Menetrey J, Llinas P, Cicolari J, Squires G, Liu X, Li A, Sweeney HL, Houdusse A, EMBO J. 2008 Jan 9;27(1):244-52. Epub 2007 Nov 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18046460 18046460] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Menetrey, J.]] | [[Category: Menetrey, J.]] | ||
[[Category: Squires, G.]] | [[Category: Squires, G.]] | ||
| - | [[Category: Sweeney, H | + | [[Category: Sweeney, H L.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: BEF]] | [[Category: BEF]] | ||
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[[Category: golgi apparatus]] | [[Category: golgi apparatus]] | ||
[[Category: membrane]] | [[Category: membrane]] | ||
| - | [[Category: mg | + | [[Category: mg adp befx]] |
[[Category: molecular motor]] | [[Category: molecular motor]] | ||
[[Category: motor protein]] | [[Category: motor protein]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:54:41 2008'' |
Revision as of 16:54, 21 February 2008
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MYOSIN VI (MD-INSERT2-CAM, DELTA-INSERT1) POST-RIGOR STATE
Overview
Myosin VI has an unexpectedly large swing of its lever arm (powerstroke) that optimizes its unique reverse direction movement. The basis for this is an unprecedented rearrangement of the subdomain to which the lever arm is attached, referred to as the converter. It is unclear at what point(s) in the myosin VI ATPase cycle rearrangements in the converter occur, and how this would effect lever arm position. We solved the structure of myosin VI with an ATP analogue (ADP.BeF3) bound in its nucleotide-binding pocket. The structure reveals that no rearrangement in the converter occur upon ATP binding. Based on previously solved myosin structures, our structure suggests that no reversal of the powerstroke occurs during detachment of myosin VI from actin. The structure also reveals novel features of the myosin VI motor that may be important in maintaining the converter conformation during detachment from actin, and other features that may promote rapid rearrangements in the structure following actin detachment that enable hydrolysis of ATP.
About this Structure
2VAS is a Protein complex structure of sequences from Drosophila melanogaster and Sus scrofa with , , and as ligands. Known structural/functional Sites: , , , , , and . Full crystallographic information is available from OCA.
Reference
The post-rigor structure of myosin VI and implications for the recovery stroke., Menetrey J, Llinas P, Cicolari J, Squires G, Liu X, Li A, Sweeney HL, Houdusse A, EMBO J. 2008 Jan 9;27(1):244-52. Epub 2007 Nov 29. PMID:18046460
Page seeded by OCA on Thu Feb 21 18:54:41 2008
Categories: Drosophila melanogaster | Protein complex | Sus scrofa | Cicolari, J. | Houdusse, A. | Li, A. | Liu, X. | Llinas, P. | Menetrey, J. | Squires, G. | Sweeney, H L. | ADP | BEF | CA | MG | Actin-binding | Atp-binding | Calmodulin | Calmodulin-binding | Cam | Coiled coil | Cytoplasm | Endocytosis | Golgi apparatus | Membrane | Mg adp befx | Molecular motor | Motor protein | Myosin | Myosin vi | Nucleotide-binding | Nucleus | Phosphorylation | Post-rigor state | Protein transport | Transport
