1wuv
From Proteopedia
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==Overview== | ==Overview== | ||
| - | BACKGROUND: Lectins are mainly described as simple carbohydrate-binding | + | BACKGROUND: Lectins are mainly described as simple carbohydrate-binding proteins. Previous studies have tried to identify other binding sites, which possible recognize plant hormones, secondary metabolites, and isolated amino acid residues. We report the crystal structure of a lectin isolated from Canavalia gladiata seeds (CGL), describing a new binding pocket, which may be related to pathogen resistance activity in ConA-like lectins; a site where a non-protein amino-acid, alpha-aminobutyric acid (Abu), is bound. RESULTS: The overall structure of native CGL and complexed with alpha-methyl-mannoside and Abu have been refined at 2.3 A and 2.31 A resolution, respectively. Analysis of the electron density maps of the CGL structure shows clearly the presence of Abu, which was confirmed by mass spectrometry. CONCLUSION: The presence of Abu in a plant lectin structure strongly indicates the ability of lectins on carrying secondary metabolites. Comparison of the amino acids composing the site with other legume lectins revealed that this site is conserved, providing an evidence of the biological relevance of this site. This new action of lectins strengthens their role in defense mechanisms in plants. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Canduri, F.]] | [[Category: Canduri, F.]] | ||
| - | [[Category: Cardoso, A | + | [[Category: Cardoso, A L.H.]] |
| - | [[Category: Cavada, B | + | [[Category: Cavada, B S.]] |
[[Category: Delatorre, P.]] | [[Category: Delatorre, P.]] | ||
| - | [[Category: Freitas, B | + | [[Category: Freitas, B T.]] |
| - | [[Category: Jr., W | + | [[Category: Jr., W F.Azevedo.]] |
| - | [[Category: Moreno, F | + | [[Category: Moreno, F B.M B.]] |
| - | [[Category: Rocha, B | + | [[Category: Rocha, B A.M.]] |
| - | [[Category: Sampaio, A | + | [[Category: Sampaio, A H.]] |
| - | [[Category: Souza, E | + | [[Category: Souza, E P.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: plant protein]] | [[Category: plant protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:48:21 2008'' |
Revision as of 13:48, 21 February 2008
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Crystal structure of native Canavalia gladiata lectin (CGL): a tetrameric ConA-like lectin
Overview
BACKGROUND: Lectins are mainly described as simple carbohydrate-binding proteins. Previous studies have tried to identify other binding sites, which possible recognize plant hormones, secondary metabolites, and isolated amino acid residues. We report the crystal structure of a lectin isolated from Canavalia gladiata seeds (CGL), describing a new binding pocket, which may be related to pathogen resistance activity in ConA-like lectins; a site where a non-protein amino-acid, alpha-aminobutyric acid (Abu), is bound. RESULTS: The overall structure of native CGL and complexed with alpha-methyl-mannoside and Abu have been refined at 2.3 A and 2.31 A resolution, respectively. Analysis of the electron density maps of the CGL structure shows clearly the presence of Abu, which was confirmed by mass spectrometry. CONCLUSION: The presence of Abu in a plant lectin structure strongly indicates the ability of lectins on carrying secondary metabolites. Comparison of the amino acids composing the site with other legume lectins revealed that this site is conserved, providing an evidence of the biological relevance of this site. This new action of lectins strengthens their role in defense mechanisms in plants.
About this Structure
1WUV is a Single protein structure of sequence from Canavalia gladiata with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of a lectin from Canavalia gladiata seeds: new structural insights for old molecules., Delatorre P, Rocha BA, Souza EP, Oliveira TM, Bezerra GA, Moreno FB, Freitas BT, Santi-Gadelha T, Sampaio AH, Azevedo WF Jr, Cavada BS, BMC Struct Biol. 2007 Aug 2;7:52. PMID:17683532
Page seeded by OCA on Thu Feb 21 15:48:21 2008
