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Actin
From Proteopedia
(New page: Crystal Structure of of Native G-actin 3hbt {{STRUCTURE_3hbt| PDB=3hbt | SIZE=300| SCENE= |right|CAPTION=Native G-actin 3hbt }} Actin is ...) |
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[[Image:3hbt.png|left|200px|thumb|Crystal Structure of of Native G-actin [[3hbt]]]] | [[Image:3hbt.png|left|200px|thumb|Crystal Structure of of Native G-actin [[3hbt]]]] | ||
| - | {{STRUCTURE_3hbt| PDB=3hbt | SIZE=300| SCENE= |right|CAPTION=Native G-actin [[3hbt]] }} | + | {{STRUCTURE_3hbt| PDB=3hbt | SIZE=300| SCENE=Actin/Cv/1 |right|CAPTION=Native G-actin [[3hbt]] }} |
[[Actin]] is a protein found in all eukaryotic cells. It is the monomer of two types of filaments: microfilaments which are part of the cytoskeleton and thin filaments which are part of muscles. Three isoforms of actin are identified: | [[Actin]] is a protein found in all eukaryotic cells. It is the monomer of two types of filaments: microfilaments which are part of the cytoskeleton and thin filaments which are part of muscles. Three isoforms of actin are identified: | ||
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=== Alpha actin 1 (Aa) === | === Alpha actin 1 (Aa) === | ||
| - | [[2w49]] – cAa +troponin C+ troponin T+ troponin I+ tropomyocin alpha-1 – chicken | + | [[2w49]] – cAa +troponin C+ troponin T+ troponin I+ tropomyocin alpha-1 – chicken<br /> |
| - | [[1mdu]] – cAa+hGelsolin | + | [[1mdu]] – cAa+hGelsolin<br />[[3hbt]], [[2zwh]], [[1nwk]], [[1j6z]] – rAa – rabbit<br /> |
| - | [[3hbt]], [[2zwh]], [[1nwk]], [[1j6z]] – rAa – rabbit | + | [[2q1n]], [[2q31]], [[2a5x]], [[1rdw]], [[1rfq]], [[1lcu]] – rAa – dimer<br /> |
| - | [[2q1n]], [[2q31]], [[2a5x]], [[1rdw]], [[1rfq]], [[1lcu]] – rAa – dimer | + | [[2q36]], [[1qz5]] – rAa+kabiramide C – dimer<br /> |
| - | [[2q36]], [[1qz5]] – rAa+kabiramide C – dimer | + | [[2hmp]] – rAa – protease cleaved<br /> |
| - | [[2hmp]] – rAa – protease cleaved | + | [[2zwh]], [[2gwj]], [[2gwk]] – rAa-ATP<br /> |
| - | 2zwh, 2gwj, 2gwk – rAa-ATP | + | [[2vyp]] – rAa+myxobacterial rhizopodin<br /> |
| - | 2vyp – rAa+myxobacterial rhizopodin | + | [[2v51]], [[2v52]] – rAa+mMKL/Myocardin-like protein – mouse (m)<br /> |
| - | 2v51, 2v52 – rAa+mMKL/Myocardin-like protein – mouse (m) | + | [[2vcp]] – rAa+ neural wiskott-aldrich syndrome protein (hWASP) – human (h)<br /> |
| - | 2vcp – rAa+ neural wiskott-aldrich syndrome protein (hWASP) – human (h) | + | [[2a3z]], [[2a40]], [[2a41]] – rAa+cDNase I+domains of hWASP – cow (c)<br /> |
| - | 2a3z, 2a40, 2a41 – rAa+cDNase I+domains of hWASP – cow (c) | + | [[2ff3]] - rAa+ hGelsolin +domains of hWASP <br /> |
| - | 2ff3 - rAa+ hGelsolin +domains of hWASP | + | [[2a42]] – rAa+cDNase I<br /> |
| - | 2a42 – rAa+cDNase I | + | [[3daw]] – rAa+mTwinfilin-1<br /> |
| - | 3daw – rAa+mTwinfilin-1 | + | [[3buz]] – rAa+Iota toxin component IA<br /> |
| - | 3buz – rAa+Iota toxin component IA | + | [[3b5u]] – rAa filament – fiber diffraction<br /> |
| - | 3b5u – rAa filament – fiber diffraction | + | [[1eqy]], [[3cjb]], [[1p8z]] – rAa+hGelsolin<br /> |
| - | 1eqy, 3cjb, 1p8z – rAa+hGelsolin | + | [[1rgi]] - rAa+hoGelsolin – horse (ho)<br /> |
| - | 1rgi - rAa+hoGelsolin – horse (ho) | + | [[1t44]] - rAa+hGelsolin:mThymosin B4 <br /> |
| - | 1t44 - rAa+hGelsolin:mThymosin B4 | + | [[1esv]] - rAa+hGelsolin+ latrunculin A<br /> |
| - | 1esv - rAa+hGelsolin+ latrunculin A | + | [[3cjc]] - rAa+hGelsolin+cDNase I<br /> |
| - | 3cjc - rAa+hGelsolin+cDNase I | + | [[2d1k]] - rAa+hWH2 domain of MIN+cDNase I<br /> |
| - | 2d1k - rAa+hWH2 domain of MIN+cDNase I | + | [[2pbd]] – rAa+hProfilin-1<br /> |
| - | 2pbd – rAa+hProfilin-1 | + | [[2pav]] - rAa+hProfilin-1+hVASP<br /> |
| - | 2pav - rAa+hProfilin-1+hVASP | + | [[2q97]] – rAa+toxofilin – ''Toxoplasma gondii''<br /> |
| - | 2q97 – rAa+toxofilin – Toxoplasma gondii | + | [[2q0r]] – rAa+pectenotoxin-2<br /> |
| - | 2q0r – rAa+pectenotoxin-2 | + | [[2q0u]] - rAa+pectenotoxin-2+latrunculin B<br /> |
| - | 2q0u - rAa+pectenotoxin-2+latrunculin B | + | [[1ijj]] - rAa+latrunculin A<br /> |
| - | 1ijj - rAa+latrunculin A | + | [[2fxu]] – rAa+bistramide A<br /> |
| - | 2fxu – rAa+bistramide A | + | [[1wua]] – rAa+alpyronine A<br /> |
| - | 1wua – rAa+alpyronine A | + | [[2asm]], [[2asp]] – rAa+reidispongiolide<br /> |
| - | 2asm, 2asp – rAa+reidispongiolide | + | [[2aso]] – rAa+sphinxolide B<br /> |
| - | 2aso – rAa+sphinxolide B | + | [[1yxq]] – rAa+swinholide A<br /> |
| - | 1yxq – rAa+swinholide A | + | [[1y64]] – rAa+yBNI1 domain FH2 - yeast<br /> |
| - | 1y64 – rAa+yBNI1 domain FH2 - yeast | + | [[1sqk]] – rAa+DmCiboulot - ''Drosophila melanogaster'' (Dm)<br /> |
| - | 1sqk – rAa+DmCiboulot - Drosophila melanogaster (Dm) | + | [[2ff6]] - rAa+DmCiboulot+hGelsolin <br /> |
| - | 2ff6 - rAa+DmCiboulot+hGelsolin | + | [[1s22]] – rAa+ulapualide A<br /> |
| - | 1s22 – rAa+ulapualide A | + | [[1qz6]] – rAa+jaspisamide A<br /> |
| - | 1qz6 – rAa+jaspisamide A | + | [[1lot]], [[1kxp]], [[1ma9]] – rAa+hVitamin D binding protein |
| - | 1lot, 1kxp, 1ma9 – rAa+hVitamin D binding protein | + | |
=== Beta actin (Ab) === | === Beta actin (Ab) === | ||
| - | 3lue – hAb+hAlpha-actinin | + | [[3lue]] – hAb+hAlpha-actinin <br /> |
| - | 3byh – hAb+hFimbrin ABD2 – Cryo EM | + | [[3byh]] – hAb+hFimbrin ABD2 – Cryo EM<br /> |
| - | 2oan – cAb | + | [[2oan]] – cAb |
=== Gamma 1 actin (Ag) === | === Gamma 1 actin (Ag) === | ||
| - | 1atn – rAg+cDNase I | + | [[1atn]] – rAg+cDNase I<br /> |
| - | 3chw – Major DdAg+hProfilin+poly-Pro repeat – Dictyostelium discoideum | + | [[3chw]] – Major DdAg+hProfilin+poly-Pro repeat – ''Dictyostelium discoideum''<br /> |
| - | 3ci5, 3cip, 1nmd - Major DdAg+hGelsolin | + | [[3ci5]], [[3cip]], [[1nmd]] - Major DdAg+hGelsolin<br /> |
| - | 1c0g, 1dej – DdAg:tetrahymenatA+hGelsolin | + | [[1c0g]], [[1dej]] – DdAg:tetrahymenatA+hGelsolin<br /> |
| - | 1nm1 - DdAg+hGelsolin+Mg-ATP | + | [[1nm1]] - DdAg+hGelsolin+Mg-ATP<br /> |
| - | 1nlv, 1c0f – DdAg+hGelsolin | + | [[1nlv]], [[1c0f]] – DdAg+hGelsolin<br /> |
| - | 1d4x – Ag 1/3+hGelsolin – C. elegans | + | [[1d4x]] – Ag 1/3+hGelsolin – ''C. elegans''<br /> |
| - | 1h1v – hAg+rGelsolin | + | [[1h1v]] – hAg+rGelsolin<br /> |
| - | 1hlu, 2btf – cA+cProfilin | + | [[1hlu]], [[2btf]] – cA+cProfilin<br /> |
| - | 1o1g, 1o1a | + | [[1o1g]], [[1o1a]], [[1o1b]], [[1o1c]], [[1o1d]], [[1o1e]], [[1o1f]], [[1o18]], [[1o19]], [[1mvw]], [[1m8q]] –cAg+cMyosin II - tomography<br /> |
| - | 3eks – DmAg 5c (mutant) | + | [[3eks]] – DmAg 5c (mutant)<br /> |
| - | 3eku – DmAg 5c (mutant)+cytochalasin D | + | [[3eku]] – DmAg 5c (mutant)+cytochalasin D<br /> |
| - | 3el2, 2hf4 - DmAg 5c (mutant)+Ca-ATP | + | [[3el2]], [[2hf4]] - DmAg 5c (mutant)+Ca-ATP<br /> |
| - | 2hf3 - DmAg 5c (mutant)+Ca-ADP | + | [[2hf3]] - DmAg 5c (mutant)+Ca-ADP<br /> |
| - | 3b63 – actin filament – Limulus polyphemus | + | [[3b63]] – actin filament – ''Limulus polyphemus''<br /> |
| - | 1yvn – yA (mutant)+hGelsolin | + | [[1yvn]] – yA (mutant)+hGelsolin <br /> |
| - | 1yag - yA+hGelsolin | + | [[1yag]] - yA+hGelsolin<br /> |
Revision as of 12:38, 9 December 2010
Actin is a protein found in all eukaryotic cells. It is the monomer of two types of filaments: microfilaments which are part of the cytoskeleton and thin filaments which are part of muscles. Three isoforms of actin are identified: Alpha (Aa) (or G-actin) found in muscle tissue, beta (Ab) and gamma (Ag) actins are components of the cytoskeleton. F-actin is Aa bound to ATP. The images at the left and at the right correspond to one representative Actin, i.e. the crystal structure of rabbit Native G-actin (3hbt).
3D Structures of Actin
Alpha actin 1 (Aa)
2w49 – cAa +troponin C+ troponin T+ troponin I+ tropomyocin alpha-1 – chicken
1mdu – cAa+hGelsolin
3hbt, 2zwh, 1nwk, 1j6z – rAa – rabbit
2q1n, 2q31, 2a5x, 1rdw, 1rfq, 1lcu – rAa – dimer
2q36, 1qz5 – rAa+kabiramide C – dimer
2hmp – rAa – protease cleaved
2zwh, 2gwj, 2gwk – rAa-ATP
2vyp – rAa+myxobacterial rhizopodin
2v51, 2v52 – rAa+mMKL/Myocardin-like protein – mouse (m)
2vcp – rAa+ neural wiskott-aldrich syndrome protein (hWASP) – human (h)
2a3z, 2a40, 2a41 – rAa+cDNase I+domains of hWASP – cow (c)
2ff3 - rAa+ hGelsolin +domains of hWASP
2a42 – rAa+cDNase I
3daw – rAa+mTwinfilin-1
3buz – rAa+Iota toxin component IA
3b5u – rAa filament – fiber diffraction
1eqy, 3cjb, 1p8z – rAa+hGelsolin
1rgi - rAa+hoGelsolin – horse (ho)
1t44 - rAa+hGelsolin:mThymosin B4
1esv - rAa+hGelsolin+ latrunculin A
3cjc - rAa+hGelsolin+cDNase I
2d1k - rAa+hWH2 domain of MIN+cDNase I
2pbd – rAa+hProfilin-1
2pav - rAa+hProfilin-1+hVASP
2q97 – rAa+toxofilin – Toxoplasma gondii
2q0r – rAa+pectenotoxin-2
2q0u - rAa+pectenotoxin-2+latrunculin B
1ijj - rAa+latrunculin A
2fxu – rAa+bistramide A
1wua – rAa+alpyronine A
2asm, 2asp – rAa+reidispongiolide
2aso – rAa+sphinxolide B
1yxq – rAa+swinholide A
1y64 – rAa+yBNI1 domain FH2 - yeast
1sqk – rAa+DmCiboulot - Drosophila melanogaster (Dm)
2ff6 - rAa+DmCiboulot+hGelsolin
1s22 – rAa+ulapualide A
1qz6 – rAa+jaspisamide A
1lot, 1kxp, 1ma9 – rAa+hVitamin D binding protein
Beta actin (Ab)
3lue – hAb+hAlpha-actinin
3byh – hAb+hFimbrin ABD2 – Cryo EM
2oan – cAb
Gamma 1 actin (Ag)
1atn – rAg+cDNase I
3chw – Major DdAg+hProfilin+poly-Pro repeat – Dictyostelium discoideum
3ci5, 3cip, 1nmd - Major DdAg+hGelsolin
1c0g, 1dej – DdAg:tetrahymenatA+hGelsolin
1nm1 - DdAg+hGelsolin+Mg-ATP
1nlv, 1c0f – DdAg+hGelsolin
1d4x – Ag 1/3+hGelsolin – C. elegans
1h1v – hAg+rGelsolin
1hlu, 2btf – cA+cProfilin
1o1g, 1o1a, 1o1b, 1o1c, 1o1d, 1o1e, 1o1f, 1o18, 1o19, 1mvw, 1m8q –cAg+cMyosin II - tomography
3eks – DmAg 5c (mutant)
3eku – DmAg 5c (mutant)+cytochalasin D
3el2, 2hf4 - DmAg 5c (mutant)+Ca-ATP
2hf3 - DmAg 5c (mutant)+Ca-ADP
3b63 – actin filament – Limulus polyphemus
1yvn – yA (mutant)+hGelsolin
1yag - yA+hGelsolin
