2qv2
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Mutations in the inositol 5-phosphatase OCRL are responsible for Lowe | + | Mutations in the inositol 5-phosphatase OCRL are responsible for Lowe syndrome, whose manifestations include mental retardation and renal Fanconi syndrome. OCRL has been implicated in membrane trafficking, but disease mechanisms remain unclear. We show that OCRL visits late-stage, endocytic clathrin-coated pits and binds the Rab5 effector APPL1 on peripheral early endosomes. The interaction with APPL1, which is mediated by the ASH-RhoGAP-like domains of OCRL and is abolished by disease mutations, provides a link to protein networks implicated in the reabsorptive function of the kidney and in the trafficking and signaling of growth factor receptors in the brain. Crystallographic studies reveal a role of the ASH-RhoGAP-like domains in positioning the phosphatase domain at the membrane interface and a clathrin box protruding from the RhoGAP-like domain. Our results support a role of OCRL in the early endocytic pathway, consistent with the predominant localization of its preferred substrates, PI(4,5)P(2) and PI(3,4,5)P(3), at the cell surface. |
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| + | ==Disease== | ||
| + | Known diseases associated with this structure: Dent syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=300535 300535]], Lowe syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=300535 300535]] | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Phosphoinositide 5-phosphatase]] | [[Category: Phosphoinositide 5-phosphatase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Camilli, P | + | [[Category: Camilli, P De.]] |
| - | [[Category: Erdman, K | + | [[Category: Erdman, K S.]] |
[[Category: Mao, Y.]] | [[Category: Mao, Y.]] | ||
| - | [[Category: McCrea, H | + | [[Category: McCrea, H J.]] |
[[Category: appl1]] | [[Category: appl1]] | ||
[[Category: ash]] | [[Category: ash]] | ||
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[[Category: rhogap]] | [[Category: rhogap]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:42:29 2008'' |
Revision as of 16:42, 21 February 2008
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A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway
Contents |
Overview
Mutations in the inositol 5-phosphatase OCRL are responsible for Lowe syndrome, whose manifestations include mental retardation and renal Fanconi syndrome. OCRL has been implicated in membrane trafficking, but disease mechanisms remain unclear. We show that OCRL visits late-stage, endocytic clathrin-coated pits and binds the Rab5 effector APPL1 on peripheral early endosomes. The interaction with APPL1, which is mediated by the ASH-RhoGAP-like domains of OCRL and is abolished by disease mutations, provides a link to protein networks implicated in the reabsorptive function of the kidney and in the trafficking and signaling of growth factor receptors in the brain. Crystallographic studies reveal a role of the ASH-RhoGAP-like domains in positioning the phosphatase domain at the membrane interface and a clathrin box protruding from the RhoGAP-like domain. Our results support a role of OCRL in the early endocytic pathway, consistent with the predominant localization of its preferred substrates, PI(4,5)P(2) and PI(3,4,5)P(3), at the cell surface.
Disease
Known diseases associated with this structure: Dent syndrome OMIM:[300535], Lowe syndrome OMIM:[300535]
About this Structure
2QV2 is a Single protein structure of sequence from Homo sapiens. Active as Phosphoinositide 5-phosphatase, with EC number 3.1.3.36 Full crystallographic information is available from OCA.
Reference
A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway., Erdmann KS, Mao Y, McCrea HJ, Zoncu R, Lee S, Paradise S, Modregger J, Biemesderfer D, Toomre D, De Camilli P, Dev Cell. 2007 Sep;13(3):377-90. PMID:17765681
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