2v26
From Proteopedia
(New page: 200px<br /><applet load="2v26" size="350" color="white" frame="true" align="right" spinBox="true" caption="2v26, resolution 1.75Å" /> '''MYOSIN VI (MD) PRE-P...) |
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==Overview== | ==Overview== | ||
| - | Due to a unique addition to the lever arm-positioning region (converter), class VI myosins move in the opposite direction (toward the minus-end of | + | Due to a unique addition to the lever arm-positioning region (converter), class VI myosins move in the opposite direction (toward the minus-end of actin filaments) compared to other characterized myosin classes. However, the large size of the myosin VI lever arm swing (powerstroke) cannot be explained by our current view of the structural transitions that occur within the myosin motor. We have solved the crystal structure of a fragment of the myosin VI motor in the structural state that represents the starting point for movement on actin; the pre-powerstroke state. Unexpectedly, the converter itself rearranges to achieve a conformation that has not been seen for other myosins. This results in a much larger powerstroke than is achievable without the converter rearrangement. Moreover, it provides a new mechanism that could be exploited to increase the powerstroke of yet to be characterized plus-end-directed myosin classes. |
==About this Structure== | ==About this Structure== | ||
| - | 2V26 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=VO4:'>VO4</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Adp Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Mg Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Vo4 Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Edo Binding Site For Chain A'>AC4</scene>, <scene name='pdbsite=AC5:Edo Binding Site For Chain A'>AC5</scene>, <scene name='pdbsite=AC6:Edo Binding Site For Chain A'>AC6</scene>, <scene name='pdbsite=AC7:Edo Binding Site For Chain A'>AC7</scene>, <scene name='pdbsite=AC8:Edo Binding Site For Chain A'>AC8</scene>, <scene name='pdbsite=AC9:Edo Binding Site For Chain A'>AC9</scene>, <scene name='pdbsite=BC1:Edo Binding Site For Chain A'>BC1</scene>, <scene name='pdbsite=BC2:So4 Binding Site For Chain A'>BC2</scene> and <scene name='pdbsite=BC3:So4 Binding Site For Chain A'>BC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V26 OCA]. | + | 2V26 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=VO4:'>VO4</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Vo4+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Edo+Binding+Site+For+Chain+A'>AC4</scene>, <scene name='pdbsite=AC5:Edo+Binding+Site+For+Chain+A'>AC5</scene>, <scene name='pdbsite=AC6:Edo+Binding+Site+For+Chain+A'>AC6</scene>, <scene name='pdbsite=AC7:Edo+Binding+Site+For+Chain+A'>AC7</scene>, <scene name='pdbsite=AC8:Edo+Binding+Site+For+Chain+A'>AC8</scene>, <scene name='pdbsite=AC9:Edo+Binding+Site+For+Chain+A'>AC9</scene>, <scene name='pdbsite=BC1:Edo+Binding+Site+For+Chain+A'>BC1</scene>, <scene name='pdbsite=BC2:So4+Binding+Site+For+Chain+A'>BC2</scene> and <scene name='pdbsite=BC3:So4+Binding+Site+For+Chain+A'>BC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V26 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Menetrey, J.]] | [[Category: Menetrey, J.]] | ||
[[Category: Mukherjea, M.]] | [[Category: Mukherjea, M.]] | ||
| - | [[Category: Sweeney, H | + | [[Category: Sweeney, H L.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
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[[Category: vanadate]] | [[Category: vanadate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:52:41 2008'' |
Revision as of 16:52, 21 February 2008
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MYOSIN VI (MD) PRE-POWERSTROKE STATE (MG.ADP.VO4)
Overview
Due to a unique addition to the lever arm-positioning region (converter), class VI myosins move in the opposite direction (toward the minus-end of actin filaments) compared to other characterized myosin classes. However, the large size of the myosin VI lever arm swing (powerstroke) cannot be explained by our current view of the structural transitions that occur within the myosin motor. We have solved the crystal structure of a fragment of the myosin VI motor in the structural state that represents the starting point for movement on actin; the pre-powerstroke state. Unexpectedly, the converter itself rearranges to achieve a conformation that has not been seen for other myosins. This results in a much larger powerstroke than is achievable without the converter rearrangement. Moreover, it provides a new mechanism that could be exploited to increase the powerstroke of yet to be characterized plus-end-directed myosin classes.
About this Structure
2V26 is a Single protein structure of sequence from Sus scrofa with , , , and as ligands. Known structural/functional Sites: , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
The structural basis for the large powerstroke of myosin VI., Menetrey J, Llinas P, Mukherjea M, Sweeney HL, Houdusse A, Cell. 2007 Oct 19;131(2):300-8. PMID:17956731
Page seeded by OCA on Thu Feb 21 18:52:41 2008
Categories: Single protein | Sus scrofa | Houdusse, A. | Llinas, P. | Menetrey, J. | Mukherjea, M. | Sweeney, H L. | ADP | EDO | MG | SO4 | VO4 | Actin-binding | Atp-binding | Calmodulin-binding | Coiled coil | Domain motor | Endocytosis | Golgi apparatus | Membrane | Molecular motor | Motor protein | Myosin | Myosin vi | Nuclear protein | Nucleotide-binding | Phosphorylation | Pre- powerstroke | Protein transport | Structural protein | Transition state | Transport | Vanadate
