This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2q33
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The D-enantiomer of a potently sweet protein, monellin, has been | + | The D-enantiomer of a potently sweet protein, monellin, has been crystallized and analyzed by X-ray crystallography at 1.8 A resolut ion. Two crystal forms (I and II) appeared under crystallization conditions similar, but not identical, to the crystallization conditions of natural L-monellin. There are four molecules per asymmetric unit in crystal form I and one in crystal form II. Crystal form I is not reproducible and is equivalent to that of monoclinic L-monellin. Intermonomer contacts in crystal form II are very different from those found in natural L-monellin crystals. The backbone trace of D-monellin resembles very closely the mirror image of that of L-monellin, but the N- and C-terminus backbones as well as several side-chain conformations of D-monellin are different from those of natural L-monellin. Most of these apparent differences may be attributable to the crystal packing differences. |
==About this Structure== | ==About this Structure== | ||
| - | 2Q33 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. This structure | + | 2Q33 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. This structure supersedes the now removed PDB entry 1N98. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q33 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Ariyoshi, Y.]] | [[Category: Ariyoshi, Y.]] | ||
| - | [[Category: Hung, L | + | [[Category: Hung, L W.]] |
| - | [[Category: Kim, S | + | [[Category: Kim, S H.]] |
[[Category: Kohmura, M.]] | [[Category: Kohmura, M.]] | ||
[[Category: all-d protein]] | [[Category: all-d protein]] | ||
| Line 20: | Line 20: | ||
[[Category: de novo protein]] | [[Category: de novo protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:35:25 2008'' |
Revision as of 16:35, 21 February 2008
|
Crystal structure of all-D monellin at 1.8 A resolution
Overview
The D-enantiomer of a potently sweet protein, monellin, has been crystallized and analyzed by X-ray crystallography at 1.8 A resolut ion. Two crystal forms (I and II) appeared under crystallization conditions similar, but not identical, to the crystallization conditions of natural L-monellin. There are four molecules per asymmetric unit in crystal form I and one in crystal form II. Crystal form I is not reproducible and is equivalent to that of monoclinic L-monellin. Intermonomer contacts in crystal form II are very different from those found in natural L-monellin crystals. The backbone trace of D-monellin resembles very closely the mirror image of that of L-monellin, but the N- and C-terminus backbones as well as several side-chain conformations of D-monellin are different from those of natural L-monellin. Most of these apparent differences may be attributable to the crystal packing differences.
About this Structure
2Q33 is a Protein complex structure of sequences from [1]. This structure supersedes the now removed PDB entry 1N98. Full crystallographic information is available from OCA.
Reference
Structure of an enantiomeric protein, D-monellin at 1.8 A resolution., Hung LW, Kohmura M, Ariyoshi Y, Kim SH, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):494-500. PMID:9867435
Page seeded by OCA on Thu Feb 21 18:35:25 2008
