2q33
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The D-enantiomer of a potently sweet protein, monellin, has been | + | The D-enantiomer of a potently sweet protein, monellin, has been crystallized and analyzed by X-ray crystallography at 1.8 A resolut ion. Two crystal forms (I and II) appeared under crystallization conditions similar, but not identical, to the crystallization conditions of natural L-monellin. There are four molecules per asymmetric unit in crystal form I and one in crystal form II. Crystal form I is not reproducible and is equivalent to that of monoclinic L-monellin. Intermonomer contacts in crystal form II are very different from those found in natural L-monellin crystals. The backbone trace of D-monellin resembles very closely the mirror image of that of L-monellin, but the N- and C-terminus backbones as well as several side-chain conformations of D-monellin are different from those of natural L-monellin. Most of these apparent differences may be attributable to the crystal packing differences. |
==About this Structure== | ==About this Structure== | ||
| - | 2Q33 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. This structure | + | 2Q33 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. This structure supersedes the now removed PDB entry 1N98. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q33 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Ariyoshi, Y.]] | [[Category: Ariyoshi, Y.]] | ||
| - | [[Category: Hung, L | + | [[Category: Hung, L W.]] |
| - | [[Category: Kim, S | + | [[Category: Kim, S H.]] |
[[Category: Kohmura, M.]] | [[Category: Kohmura, M.]] | ||
[[Category: all-d protein]] | [[Category: all-d protein]] | ||
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[[Category: de novo protein]] | [[Category: de novo protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:35:25 2008'' |
Revision as of 16:35, 21 February 2008
|
Crystal structure of all-D monellin at 1.8 A resolution
Overview
The D-enantiomer of a potently sweet protein, monellin, has been crystallized and analyzed by X-ray crystallography at 1.8 A resolut ion. Two crystal forms (I and II) appeared under crystallization conditions similar, but not identical, to the crystallization conditions of natural L-monellin. There are four molecules per asymmetric unit in crystal form I and one in crystal form II. Crystal form I is not reproducible and is equivalent to that of monoclinic L-monellin. Intermonomer contacts in crystal form II are very different from those found in natural L-monellin crystals. The backbone trace of D-monellin resembles very closely the mirror image of that of L-monellin, but the N- and C-terminus backbones as well as several side-chain conformations of D-monellin are different from those of natural L-monellin. Most of these apparent differences may be attributable to the crystal packing differences.
About this Structure
2Q33 is a Protein complex structure of sequences from [1]. This structure supersedes the now removed PDB entry 1N98. Full crystallographic information is available from OCA.
Reference
Structure of an enantiomeric protein, D-monellin at 1.8 A resolution., Hung LW, Kohmura M, Ariyoshi Y, Kim SH, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):494-500. PMID:9867435
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