2qfa
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The chromosomal passenger complex (CPC) is a key regulator of chromosome | + | The chromosomal passenger complex (CPC) is a key regulator of chromosome segregation and cytokinesis. CPC functions are connected to its localization. The complex first localizes to centromeres and later associates with the central spindle and midbody. Survivin, Borealin, and INCENP are the three components of the CPC that regulate the activity and localization of its enzymatic component, the kinase Aurora B. We determined the 1.4 A resolution crystal structure of the regulatory core of the CPC, revealing that Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle. We used siRNA rescue experiments with structure-based mutants to explore the requirements for CPC localization. We show that the intertwined structural interactions of the core components lead to functional interdependence. Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Structure of a Survivin-Borealin-INCENP | + | Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together., Jeyaprakash AA, Klein UR, Lindner D, Ebert J, Nigg EA, Conti E, Cell. 2007 Oct 19;131(2):271-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17956729 17956729] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Conti, E.]] | [[Category: Conti, E.]] | ||
[[Category: Ebert, J.]] | [[Category: Ebert, J.]] | ||
| - | [[Category: Jeyaprakash, A | + | [[Category: Jeyaprakash, A A.]] |
| - | [[Category: Klein, U | + | [[Category: Klein, U R.]] |
[[Category: Lindner, D.]] | [[Category: Lindner, D.]] | ||
| - | [[Category: Nigg, E | + | [[Category: Nigg, E A.]] |
[[Category: MES]] | [[Category: MES]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:38:53 2008'' |
Revision as of 16:38, 21 February 2008
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Crystal structure of a Survivin-Borealin-INCENP core complex
Overview
The chromosomal passenger complex (CPC) is a key regulator of chromosome segregation and cytokinesis. CPC functions are connected to its localization. The complex first localizes to centromeres and later associates with the central spindle and midbody. Survivin, Borealin, and INCENP are the three components of the CPC that regulate the activity and localization of its enzymatic component, the kinase Aurora B. We determined the 1.4 A resolution crystal structure of the regulatory core of the CPC, revealing that Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle. We used siRNA rescue experiments with structure-based mutants to explore the requirements for CPC localization. We show that the intertwined structural interactions of the core components lead to functional interdependence. Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization.
About this Structure
2QFA is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together., Jeyaprakash AA, Klein UR, Lindner D, Ebert J, Nigg EA, Conti E, Cell. 2007 Oct 19;131(2):271-85. PMID:17956729
Page seeded by OCA on Thu Feb 21 18:38:53 2008
Categories: Homo sapiens | Protein complex | Conti, E. | Ebert, J. | Jeyaprakash, A A. | Klein, U R. | Lindner, D. | Nigg, E A. | MES | ZN | Alternative splicing | Apoptosis | Cell cycle | Cell cycle/cell cycle/cell cycle complex | Cell division | Centromere | Chromosomal protein | Coiled coil | Cytoplasm | Long helix | Metal-binding | Microtubule | Mitosis | Nucleus | Phosphorylation | Polymorphism | Protease inhibitor | Thiol protease inhibitor | Three-helical-bundle | Zinc
