2z8x
From Proteopedia
(New page: 200px<br /><applet load="2z8x" size="350" color="white" frame="true" align="right" spinBox="true" caption="2z8x, resolution 1.48Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in | + | The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Matsumura, H.]] | [[Category: Matsumura, H.]] | ||
[[Category: Takano, K.]] | [[Category: Takano, K.]] | ||
| - | [[Category: You, D | + | [[Category: You, D J.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: beta roll]] | [[Category: beta roll]] | ||
[[Category: calcium binding protein]] | [[Category: calcium binding protein]] | ||
| - | [[Category: family i | + | [[Category: family i 3 lipase]] |
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
[[Category: rtx protein]] | [[Category: rtx protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:00:50 2008'' |
Revision as of 17:00, 21 February 2008
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Crystal structure of extracellular lipase from Pseudomonas sp. MIS38
Overview
The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.
About this Structure
2Z8X is a Single protein structure of sequence from Pseudomonas sp. mis38 with and as ligands. Active as Triacylglycerol lipase, with EC number 3.1.1.3 Full crystallographic information is available from OCA.
Reference
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S, FEBS Lett. 2007 Oct 30;581(26):5060-4. Epub 2007 Oct 1. PMID:17923123
Page seeded by OCA on Thu Feb 21 19:00:50 2008
