2v9s
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 2V9S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GOL:'>GOL</scene> and <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9S OCA]. | + | 2V9S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GOL:'>GOL</scene> and <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: structural protein]] | [[Category: structural protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:50:43 2008'' |
Revision as of 08:50, 3 February 2008
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SECOND LRR DOMAIN OF HUMAN SLIT2
Overview
Slits are large multidomain leucine-rich repeat (LRR)-containing proteins, that provide crucial guidance cues in neuronal and vascular development., More recently, Slits have been implicated in heart morphogenesis, angiogenesis, and tumor metastasis. Slits are ligands for the Robo, (Roundabout) receptors, which belong to the Ig superfamily of, transmembrane signaling molecules. The Slit-Robo interaction is mediated, by the second LRR domain of Slit and the two N-terminal Ig domains of, Robo, but the molecular details of this interaction and how it induces, signaling remain unclear. Here we describe the crystal structures of the, second LRR domain of human Slit2 (Slit2 D2), the first two Ig domains of, its receptor Robo1 (Ig1-2), and the minimal complex between these proteins, (Slit2 D2-Robo1 Ig1). Slit2 D2 binds with its concave surface to the side, of Ig1 with electrostatic and hydrophobic contact regions mediated by, residues that are conserved in other family members. Surface plasmon, resonance experiments and a mutational analysis of the interface confirm, that Ig1 is the primary domain for binding Slit2. These structures provide, molecular insight into Slit-Robo complex formation and will be important, for the development of novel cancer therapeutics.
About this Structure
2V9S is a Single protein structure of sequence from Homo sapiens with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural insights into the Slit-Robo complex., Morlot C, Thielens NM, Ravelli RB, Hemrika W, Romijn RA, Gros P, Cusack S, McCarthy AA, Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14923-8. Epub 2007 Sep 11. PMID:17848514
Page seeded by OCA on Sun Feb 3 10:50:43 2008
Categories: Homo sapiens | Single protein | Cusack, S. | Mccarthy, A.A. | Morlot, C. | GOL | PEG | Alternative splicing | Chemotaxis | Development | Developmental protein | Differentiation | Egf-like domain | Glycoprotein | Leucine-rich repeat | Neurogenesis | Polymorphism | Receptor ligand | Robo1 | Secreted | Slit2 | Structural protein
