Luciferase

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[[Image:2d1s jena3dviewer image.jpg|left|220px]]
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[[Image:3iep.png|left|200px|thumb|Crystal Structure of Luciferase [[3iep]]]]
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== Introduction ==
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{{STRUCTURE_3iep| PDB=3iep | SIZE=300| SCENE= |right|CAPTION=Luciferase [[3iep]] }}
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Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
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==See Also==
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[[Luciferase]] (Luc) is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light. The images at the left and at the right correspond to one representative Luc, ''i.e.'' the crystal structure of firefly Luciferase ([[3iep]]).
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== 3D Structures of Luciferase ==
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[[3iep]], [[1lci]] – fLuc – firefly<br />
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[[3ier]] – fLuc+PEG 400<br />
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[[1ba3]] – fLuc+bromoform<br />
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[[3ies]] – fLuc+inhibitor<br />
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[[2d1q]] – LcLuc (mutant)+MgATP – ''Luciola cruciata''<br />
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[[2d1r]] - LcLuc (mutant)+oxyluciferin+AMP<br />
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[[2d1s]], [[2d1t]] - LcLuc (mutant)+intermediate analog<br />
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[[3fgc]] – VhLuc α+β chain+FMN – ''Vibrio harveyi''<br />
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[[1luc]], [[1brl]] - VhLuc α+β chain<br />
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[[1bsl]], [[1xkj]] - VhLuc β chain<br />
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[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br />
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==Additional information==
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[[Image:2d1s jena3dviewer image.jpg|left|220px]]
<applet load='2d1s' size='450' frame='true' align='right' scene='Luciferase/2d1s/1' caption='Luciferase ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}}.' />
<applet load='2d1s' size='450' frame='true' align='right' scene='Luciferase/2d1s/1' caption='Luciferase ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}}.' />
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Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
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{{Clear}}
*[[Luciola cruciata luciferase]] (Japanese firefly), complexed with Oxyluciferin & AMP ([[2d1r]]) or with an high-energy analogue ([[2d1s]])
*[[Luciola cruciata luciferase]] (Japanese firefly), complexed with Oxyluciferin & AMP ([[2d1r]]) or with an high-energy analogue ([[2d1s]])
*[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref>
*[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref>

Revision as of 09:02, 21 February 2011

Image:3iep.png
Crystal Structure of Luciferase 3iep

Template:STRUCTURE 3iep

Luciferase (Luc) is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light. The images at the left and at the right correspond to one representative Luc, i.e. the crystal structure of firefly Luciferase (3iep).

Contents







3D Structures of Luciferase

3iep, 1lci – fLuc – firefly
3ier – fLuc+PEG 400
1ba3 – fLuc+bromoform
3ies – fLuc+inhibitor
2d1q – LcLuc (mutant)+MgATP – Luciola cruciata
2d1r - LcLuc (mutant)+oxyluciferin+AMP
2d1s, 2d1t - LcLuc (mutant)+intermediate analog
3fgc – VhLuc α+β chain+FMN – Vibrio harveyi
1luc, 1brl - VhLuc α+β chain
1bsl, 1xkj - VhLuc β chain
2psd, 2psh, 2psj, 2pse, 2psf – Luc (mutant) – Renilla reniformis


Additional information

Luciferase (2d1s) <jmol> 

<jmolLink>
 <script>if (~fancyoff); set ribbonborder off; set antialiasdisplay off;~fancyoff=false; else; set ribbonborder on; set antialiasdisplay on; select protein; cartoon on; ~fancyoff=true; endif;
 </script>
 <text>Fancy, high-quality cartoons on/off</text>

<!--Makes it look like fancy images at and from Jena Library --> 

</jmolLink>

</jmol>.

Drag the structure with the mouse to rotate

Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.

  • Luciola cruciata luciferase (Japanese firefly), complexed with Oxyluciferin & AMP (2d1r) or with an high-energy analogue (2d1s)
  • 3ies,3iep and 3ier Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124[1]
  • 1ba3 firefly luciferase enzyme from Photinus pyralis
  • 2psd, 2pse, 2psf, 2psh, 2psj, and 2rh7 the luciferase from Renilla reniformis (RLuc)
  • 1bsl 1.95Å heterodimer of bacterial luciferase from Vibrio harveyi
  • 1brl 2.4Å heterodimer of bacterial luciferase from Vibrio harveyi
  • 1xkj homodimer of bacterial luciferase from Vibrio harveyi
  • 2d1q, 2d1r and 2d1s thermostable Japanese Firefly (Luciola cruciata) Luciferase complexed with High-energy intermediate analog
  • 2d1t thermostable Japanese Firefly Luciferase red-color emission S286N mutant
  • 1lci firefly luciferase enzyme from Photinus pyralis
  • Dinoflagellate luciferase
  • 1luc The 1.5Å resolution crystal structure of bacterial luciferase from Vibrio harveyi
  • 3fgc Bacterial luciferase from Vibrio harveyi[2]
  • 1vpr luciferase of Lingulodinium polyedrum, a marine bioluminescent dinoflagellate
  • Green Fluorescent Protein
  • For additional information, see: Colored & Bioluminescent Proteins

References

  1. ↑ Auld DS, Lovell S, Thorne N, Lea WA, Maloney DJ, Shen M, Rai G, Battaile KP, Thomas CJ, Simeonov A, Hanzlik RP, Inglese J. Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124. Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4878-83. Epub 2010 Mar 1. PMID:20194791
  2. ↑ Campbell ZT, Weichsel A, Montfort WR, Baldwin TO. Crystal Structure of the Bacterial Luciferase:Flavin Complex Provides Insight into the Function of the subunit. Biochemistry. 2009 May 12. PMID:19435287 doi:http://dx.doi.org/10.1021/bi900003t


Additional Literature

  • Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
  • Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8


External Resources

Retrieved from "http://52.214.119.220/wiki/index.php/Luciferase"
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