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1aii

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[[Category: phospholipase a2 inhibitor]]
[[Category: phospholipase a2 inhibitor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:32:49 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:48:43 2007''

Revision as of 12:44, 30 October 2007


1aii, resolution 1.95Å

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ANNEXIN III

Overview

Annexin III, a putative inositol (1,2)-phosphohydrolase, was, co-crystallized with inositol 2-phosphate, the inhibitor of the reaction, and its structure was solved to 1.95 A resolution. No enzyme active site, was observed in the structure. Assays for enzymatic activity were also, negative. Search for annexin III-inositol phosphate interactions using the, BIAcoreTM system revealed an affinity for inositol cyclic (1,2)-phosphate, suggesting annexin III may sequester the molecule in the cell. The, BIAcoreTM system used with different phospholipids showed that annexin III, displays specificity for phosphatidylethanolamine, but not for, phosphatidylinositols. Interestingly, a molecule of ethanolamine was found, bound to the protein in the crystal structure. Coupled with the fact that, this is ... [(full description)]

About this Structure

1AII is a [Single protein] structure of sequence from [Homo sapiens] with CA, SO4 and ETA as [ligands]. Structure known Active Sites: CAD, CBD and CCD. Full crystallographic information is available from [OCA].

Reference

Can enzymatic activity, or otherwise, be inferred from structural studies of annexin III?, Perron B, Lewit-Bentley A, Geny B, Russo-Marie F, J Biol Chem. 1997 Apr 25;272(17):11321-6. PMID:9111038

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