2j5g

From Proteopedia

Revision as of 15:19, 30 October 2007

THE NATIVE STRUCTURE OF A BETA-DIKETONE HYDROLASE FROM THE CYANOBACTERIUM ANABAENA SP. PCC 7120

Overview

The gene alr4455 from the well-studied cyanobacterium Anabaena sp. PCC, 7120 encodes a crotonase orthologue that displays beta-diketone hydrolase, activity. Anabaena beta-diketone hydrolase (ABDH), in common with, 6-oxocamphor hydrolase (OCH) from Rhodococcus sp. NCIMB 9784, catalyzes, the desymmetrization of bicyclo[2.2.2]octane-2,6-dione to yield, [(S)-3-oxocyclohexyl]acetic acid, a reaction unusual among the crotonase, superfamily as the substrate is not an acyl-CoA thioester. The structure, of ABDH has been determined to a resolution of 1.5 A in both native and, ligand-bound forms. ABDH forms a hexamer similar to OCH and features one, active site per enzyme monomer. The arrangement of side chains in the, active site indicates that while the catalytic chemistry may be conserved, in OCH ... [(full description)]

About this Structure

2J5G is a [Single protein] structure of sequence from [Anabaena sp.] with SO4 as [ligand]. Active as [Beta-diketone hydrolase], with EC number [3.7.1.7]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural characterization of a beta-diketone hydrolase from the cyanobacterium Anabaena sp. PCC 7120 in native and product-bound forms, a coenzyme A-independent member of the crotonase suprafamily., Bennett JP, Whittingham JL, Brzozowski AM, Leonard PM, Grogan G, Biochemistry. 2007 Jan 9;46(1):137-44. PMID:17198383

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