2qow
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The widely used antibiotic spectinomycin inhibits bacterial protein | + | The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
| - | [[Category: Borovinskaya, M | + | [[Category: Borovinskaya, M A.]] |
| - | [[Category: Cate, J | + | [[Category: Cate, J H.D.]] |
[[Category: Fredrick, K.]] | [[Category: Fredrick, K.]] | ||
| - | [[Category: Holton, J | + | [[Category: Holton, J M.]] |
[[Category: Shoji, S.]] | [[Category: Shoji, S.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: trna-binding]] | [[Category: trna-binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:00 2008'' |
Revision as of 16:41, 21 February 2008
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Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.
Overview
The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.
About this Structure
2QOW is a Protein complex structure of sequences from Escherichia coli and Salmonella typhimurium with and as ligands. Full crystallographic information is available from OCA.
Reference
A steric block in translation caused by the antibiotic spectinomycin., Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH, ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316
Page seeded by OCA on Thu Feb 21 18:41:00 2008
Categories: Escherichia coli | Protein complex | Salmonella typhimurium | Borovinskaya, M A. | Cate, J H.D. | Fredrick, K. | Holton, J M. | Shoji, S. | MG | SCM | Acetylation | Antibiotic resistance | Endonuclease | Hydrolase | Methylation | Nuclease | Repressor | Ribonucleoprotein | Ribosomal protein | Ribosome | Rna-binding | Rna-protein complex | Rrna-binding | Transcription | Transcription regulation | Transcription termination | Translation regulation | Trna-binding
