1gov
From Proteopedia
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Revision as of 13:11, 30 October 2007
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RIBONUCLEASE BI(G SPECIFIC ENDONUCLEASE) COMPLEXED WITH SULFATE IONS
Overview
The structures of Bacillus intermedius ribonuclease (binase), an, extracellular 109-residue enzyme, and its complexes with 3'GMP and sulfate, ions were solved at 1.65 and 2.0 A, respectively. The structures were, refined using REFMAC. The crystal of free binase belongs to the space, group C2, whereas the crystals of complexes belong to the space group, P2(1)2(1)2(1). In both crystal lattices the asymmetric unit contains two, molecules which form an identical dimer. The structure of the dimer is, such that only one of its subunits can bind the nucleotide in the, 3'GMP-binase complex, where the guanyl base is located in the recognition, loop of the enzyme. In both binase complex structures the phosphate group, of 3'GMP or one of the sulfate ions make an electrostatic interaction with, the ... [(full description)]
About this Structure
1GOV is a [Single protein] structure of sequence from [Bacillus intermedius] with SO4 as [ligand]. Active as [Ribonuclease T(1)], with EC number [3.1.27.3]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The structure of substrate-free microbial ribonuclease binase and of its complexes with 3'GMP and sulfate ions., Polyakov KM, Lebedev AA, Okorokov AL, Panov KI, Schulga AA, Pavlovsky AG, Karpeisky MY, Dodson GG, Acta Crystallogr D Biol Crystallogr. 2002 May;58(Pt 5):744-50. Epub 2002, Apr 26. PMID:11976484
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