2iwm
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The crystallization of three catalytically inactive mutants of penicillin | + | The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta | + | Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16508111 16508111] |
[[Category: Lysinibacillus sphaericus]] | [[Category: Lysinibacillus sphaericus]] | ||
[[Category: Penicillin amidase]] | [[Category: Penicillin amidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chandra, P | + | [[Category: Chandra, P M.]] |
[[Category: Dodson, G.]] | [[Category: Dodson, G.]] | ||
| - | [[Category: Suresh, C | + | [[Category: Suresh, C G.]] |
[[Category: antibiotic resistance]] | [[Category: antibiotic resistance]] | ||
[[Category: autoproteolysis]] | [[Category: autoproteolysis]] | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:56:45 2008'' |
Revision as of 15:56, 21 February 2008
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PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS
Overview
The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.
About this Structure
2IWM is a Single protein structure of sequence from Lysinibacillus sphaericus. Active as Penicillin amidase, with EC number 3.5.1.11 Full crystallographic information is available from OCA.
Reference
Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:16508111
Page seeded by OCA on Thu Feb 21 17:56:45 2008
