Sandbox CsrA

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==Aligned CsrA structures and Vibrio Model==
==Aligned CsrA structures and Vibrio Model==
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<Structure load='1vpz' size='500' frame='true' align='right' caption='CsRA structures aligned' scene='Sandbox_CsrA/Structuresplusmodel/3' />
 
<table style="background-color:#ffffc0" cellpadding="8" width="95%" border="0"><tr><td valign='top'>[[Image:Note.gif|left]] '''Theoretical Model:'''
<table style="background-color:#ffffc0" cellpadding="8" width="95%" border="0"><tr><td valign='top'>[[Image:Note.gif|left]] '''Theoretical Model:'''
The ''Vibrio'' structure described in this section was determined [[theoretical models|theoretically]], and hence should be interpreted with caution.</td></tr></table>
The ''Vibrio'' structure described in this section was determined [[theoretical models|theoretically]], and hence should be interpreted with caution.</td></tr></table>
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<Structure load='1vpz' size='500' frame='true' align='right' caption='CsRA structures aligned' scene='Sandbox_CsrA/Structuresplusmodel/3' />
<scene name='Sandbox_CsrA/Structuresplusmodel/4'>backbone trace of aligned structures and model</scene>.
<scene name='Sandbox_CsrA/Structuresplusmodel/4'>backbone trace of aligned structures and model</scene>.

Revision as of 14:46, 14 March 2011

Contents

CsrA

CsRA (Pseudomonas aeruginosa)

Drag the structure with the mouse to rotate

Residues 24-27 form a [1].



Residues Highlighted by Alanine Scanning Mutation of CsrA[2]





 


 


 


 


 


 


 


 


E. coli CsrA

CsRA (E. coli)

Drag the structure with the mouse to rotate


Residues Highlighted by Alanine Scanning Mutation of CsrA[3]





 


 


 


 


 


 


 


 


Aligned CsrA structures

CsRA structures aligned

Drag the structure with the mouse to rotate


Display .


Residues Highlighted by Alanine Scanning Mutation of CsrA[4]




 


 


 


 


 


 


 


 


Model of Vibrio CsrA

Theoretical Model: The protein structure described in this section was determined theoretically, and hence should be interpreted with caution.

CsRA (E. coli)

Drag the structure with the mouse to rotate


Residues Highlighted by Alanine Scanning Mutation of CsrA[5]






 


 


 


 


 


 


 


 



Aligned CsrA structures and Vibrio Model

Theoretical Model: The Vibrio structure described in this section was determined theoretically, and hence should be interpreted with caution.

CsRA structures aligned

Drag the structure with the mouse to rotate

.


Residues Highlighted by Alanine Scanning Mutation of CsrA[6]




Structures Used On This Page

1y00 is a 2 chains structure of sequences from Escherichia coli. Full experimental information is available from OCA.

1vpz is a 2 chains structure of sequences from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

2bti is a 2 chains structure of sequences from Yersinia enterocolitica. Full crystallographic information is available from OCA.

See Also

1t3o - NMR structure of Bacillus subtilis CsrA

References

  1. ↑ Rife C, Schwarzenbacher R, McMullan D, Abdubek P, Ambing E, Axelrod H, Biorac T, Canaves JM, Chiu HJ, Deacon AM, DiDonato M, Elsliger MA, Godzik A, Grittini C, Grzechnik SK, Hale J, Hampton E, Han GW, Haugen J, Hornsby M, Jaroszewski L, Klock HE, Koesema E, Kreusch A, Kuhn P, Lesley SA, Miller MD, Moy K, Nigoghossian E, Paulsen J, Quijano K, Reyes R, Sims E, Spraggon G, Stevens RC, van den Bedem H, Velasquez J, Vincent J, White A, Wolf G, Xu Q, Hodgson KO, Wooley J, Wilson IA. Crystal structure of the global regulatory protein CsrA from Pseudomonas putida at 2.05 A resolution reveals a new fold. Proteins. 2005 Nov 1;61(2):449-53. PMID:16104018 doi:10.1002/prot.20502
  2. ↑ Mercante J, Suzuki K, Cheng X, Babitzke P, Romeo T. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J Biol Chem. 2006 Oct 20;281(42):31832-42. Epub 2006 Aug 21. PMID:16923806 doi:10.1074/jbc.M606057200
  3. ↑ Mercante J, Suzuki K, Cheng X, Babitzke P, Romeo T. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J Biol Chem. 2006 Oct 20;281(42):31832-42. Epub 2006 Aug 21. PMID:16923806 doi:10.1074/jbc.M606057200
  4. ↑ Mercante J, Suzuki K, Cheng X, Babitzke P, Romeo T. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J Biol Chem. 2006 Oct 20;281(42):31832-42. Epub 2006 Aug 21. PMID:16923806 doi:10.1074/jbc.M606057200
  5. ↑ Mercante J, Suzuki K, Cheng X, Babitzke P, Romeo T. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J Biol Chem. 2006 Oct 20;281(42):31832-42. Epub 2006 Aug 21. PMID:16923806 doi:10.1074/jbc.M606057200
  6. ↑ Mercante J, Suzuki K, Cheng X, Babitzke P, Romeo T. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J Biol Chem. 2006 Oct 20;281(42):31832-42. Epub 2006 Aug 21. PMID:16923806 doi:10.1074/jbc.M606057200
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