2hhc
From Proteopedia
(New page: 200px<br /><applet load="2hhc" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hhc, resolution 1.54Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | The alpha-1,6-fucosyltransferase NodZ from Bradyrhizobium sp. WM9 | + | The alpha-1,6-fucosyltransferase NodZ from Bradyrhizobium sp. WM9 (Lupinus), composed of 325 amino acids with a molecular weight of 37 kDa, has been cloned, expressed and purified. Protein crystals suitable for X-ray diffraction were obtained under optimized crystallization conditions using ammonium dihydrogen phosphate as a precipitant. The crystals are hexagonal and belong to space group P6(1)22 or P6(5)22, with unit-cell parameters a = 125.5, c = 95.6 A, and contain 56.8% solvent and a single protein molecule in the asymmetric unit. Native data were collected to 2.85 A using synchrotron radiation and cryogenic conditions. The native crystals were soaked in a mother-liquor solution containing 2.5 mM [Ta(6)Br(12)](2+) cluster for derivatization and SAD data were collected to 3.4 A at the tantalum L(III) absorption peak. |
==About this Structure== | ==About this Structure== | ||
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[[Category: nodz]] | [[Category: nodz]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:41:53 2008'' |
Revision as of 15:41, 21 February 2008
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Crystal structure of fucosyltransferase NodZ from Bradyrhizobium
Overview
The alpha-1,6-fucosyltransferase NodZ from Bradyrhizobium sp. WM9 (Lupinus), composed of 325 amino acids with a molecular weight of 37 kDa, has been cloned, expressed and purified. Protein crystals suitable for X-ray diffraction were obtained under optimized crystallization conditions using ammonium dihydrogen phosphate as a precipitant. The crystals are hexagonal and belong to space group P6(1)22 or P6(5)22, with unit-cell parameters a = 125.5, c = 95.6 A, and contain 56.8% solvent and a single protein molecule in the asymmetric unit. Native data were collected to 2.85 A using synchrotron radiation and cryogenic conditions. The native crystals were soaked in a mother-liquor solution containing 2.5 mM [Ta(6)Br(12)](2+) cluster for derivatization and SAD data were collected to 3.4 A at the tantalum L(III) absorption peak.
About this Structure
2HHC is a Single protein structure of sequence from Bradyrhizobium sp. with and as ligands. Active as Glycoprotein 6-alpha-L-fucosyltransferase, with EC number 2.4.1.68 Full crystallographic information is available from OCA.
Reference
Cloning, purification, crystallization and preliminary crystallographic studies of Bradyrhizobium fucosyltransferase NodZ., Brzezinski K, Rogozinski B, Stepkowski T, Bujacz G, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):344-6. Epub 2004, Jan 23. PMID:14747720
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