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2uvu
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 2UVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=DGT:'>DGT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Known structural/functional Site: <scene name='pdbsite=AC1:Ca Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVU OCA]. | + | 2UVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=DGT:'>DGT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Known structural/functional Site: <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: translesion dna polymerase]] | [[Category: translesion dna polymerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:47:30 2008'' |
Revision as of 08:47, 3 February 2008
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CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS
Overview
Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) has been shown to, catalyze bypass of 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) in a highly, efficient and relatively accurate manner. Crystal structures have revealed, a potential role for Arg(332) in stabilizing the anti conformation of the, 8-oxoG template base by means of a hydrogen bond or ion-dipole pair, which, results in an increased enzymatic efficiency for dCTP insertion and makes, formation of a Hoogsteen pair between 8-oxoG and dATP less favorable., Site-directed mutagenesis was used to replace Arg(332) with Ala, Glu, Leu, or His in order to probe the importance of Arg(332) in accurate and, efficient bypass of 8-oxoG. The double mutant Ala(331)Ala(332) was also, prepared to address the contribution of Arg(331). Transientstate kinetic, results suggest that Glu(332) retains fidelity against bypass of 8-oxoG, that is similar to wild type Dpo4, a result that was confirmed by tandem, mass spectrometric analysis of full-length extension products. A crystal, structure of the Dpo4 Glu(332) mutant and 8-oxoG:C pair revealed, water-mediated hydrogen bonds between Glu(332) and the O-8 atom of 8-oxoG., The space normally occupied by Arg(332) side chain is empty in the crystal, structures of the Ala(332) mutant. Two other crystal structures show that, a Hoogsteen base pair is formed between 8-oxoG and A in the active site of, both Glu(332) and Ala(332) mutants. These results support the view that a, bond between Arg(332) and 8-oxoG plays a role in determining the fidelity, and efficiency of Dpo4-catalyzed bypass of the lesion.
About this Structure
2UVU is a Single protein structure of sequence from Sulfolobus solfataricus with and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4., Eoff RL, Irimia A, Angel KC, Egli M, Guengerich FP, J Biol Chem. 2007 Jul 6;282(27):19831-43. Epub 2007 Apr 27. PMID:17468100
Page seeded by OCA on Sun Feb 3 10:47:30 2008
Categories: DNA-directed DNA polymerase | Single protein | Sulfolobus solfataricus | Egli, M. | Irimia, A. | CA | DGT | 7 | 8-dihydro-8-oxodeoxyguanosine | Dna damage | Dna repair | Dna replication | Dna- binding | Dna-binding | Dna-directed dna polymerase | Magnesium | Metal- binding | Metal-binding | Mutator protein | Nucleotidyltransferase | P2 dna polymerase iv | Transferase | Translesion dna polymerase
