2v0o
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 2V0O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Act Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V0O OCA]. | + | 2V0O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+C'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V0O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: vesicle trafficking]] | [[Category: vesicle trafficking]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:49:04 2008'' |
Revision as of 08:49, 3 February 2008
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FCHO2 F-BAR DOMAIN
Overview
A spectrum of membrane curvatures exists within cells, and proteins have, evolved different modules to detect, create, and maintain these, curvatures. Here we present the crystal structure of one such module found, within human FCHo2. This F-BAR (extended FCH) module consists of two F-BAR, domains, forming an intrinsically curved all-helical antiparallel dimer, with a K(d) of 2.5 muM. The module binds liposomes via a concave face, deforming them into tubules with variable diameters of up to 130 nm. Pulse, EPR studies showed the membrane-bound dimer is the same as the crystal, dimer, although the N-terminal helix changed conformation on membrane, binding. Mutation of a phenylalanine on this helix partially attenuated, narrow tubule formation, and resulted in a gain of curvature sensitivity., This structure shows a distant relationship to curvature-sensing BAR, modules, and suggests how similar coiled-coil architectures in the BAR, superfamily have evolved to expand the repertoire of membrane-sculpting, possibilities.
About this Structure
2V0O is a Single protein structure of sequence from Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure and Analysis of FCHo2 F-BAR Domain: A Dimerizing and Membrane Recruitment Module that Effects Membrane Curvature., Henne WM, Kent HM, Ford MG, Hegde BG, Daumke O, Butler PJ, Mittal R, Langen R, Evans PR, McMahon HT, Structure. 2007 Jul;15(7):839-52. Epub 2007 Jun 1. PMID:17540576
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