2uy1
From Proteopedia
(New page: 200px<br /><applet load="2uy1" size="350" color="white" frame="true" align="right" spinBox="true" caption="2uy1, resolution 2.00Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The cleavage stimulation factor (CstF) is essential for the first step of | + | The cleavage stimulation factor (CstF) is essential for the first step of poly(A) tail formation at the 3' ends of mRNAs. This heterotrimeric complex is built around the 77-kDa protein bridging both CstF-64 and CstF-50 subunits. We have solved the crystal structure of the 77-kDa protein from Encephalitozoon cuniculi at a resolution of 2 A. The structure folds around 11 Half-a-TPR repeats defining two domains. The crystal structure reveals a tight homodimer exposing phylogenetically conserved areas for interaction with protein partners. Mapping experiments identify the C-terminal region of Rna14p, the yeast counterpart of CstF-77, as the docking domain for Rna15p, the yeast CstF-64 homologue. |
==About this Structure== | ==About this Structure== | ||
| Line 19: | Line 19: | ||
[[Category: rna-binding protein]] | [[Category: rna-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:51:31 2008'' |
Revision as of 16:51, 21 February 2008
|
CRYSTAL STRUCTURE OF CSTF-77
Overview
The cleavage stimulation factor (CstF) is essential for the first step of poly(A) tail formation at the 3' ends of mRNAs. This heterotrimeric complex is built around the 77-kDa protein bridging both CstF-64 and CstF-50 subunits. We have solved the crystal structure of the 77-kDa protein from Encephalitozoon cuniculi at a resolution of 2 A. The structure folds around 11 Half-a-TPR repeats defining two domains. The crystal structure reveals a tight homodimer exposing phylogenetically conserved areas for interaction with protein partners. Mapping experiments identify the C-terminal region of Rna14p, the yeast counterpart of CstF-77, as the docking domain for Rna15p, the yeast CstF-64 homologue.
About this Structure
2UY1 is a Single protein structure of sequence from Encephalitozoon cuniculi. Full crystallographic information is available from OCA.
Reference
The structure of the CstF-77 homodimer provides insights into CstF assembly., Legrand P, Pinaud N, Minvielle-Sebastia L, Fribourg S, Nucleic Acids Res. 2007;35(13):4515-22. Epub 2007 Jun 21. PMID:17584787
Page seeded by OCA on Thu Feb 21 18:51:31 2008
