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2p2r
From Proteopedia
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==Overview== | ==Overview== | ||
| - | KH (hnRNP K homology) domains, consisting of approximately 70 amino acid | + | KH (hnRNP K homology) domains, consisting of approximately 70 amino acid residues, are present in a variety of nucleic-acid-binding proteins. Among these are poly(C)-binding proteins (PCBPs), which are important regulators of mRNA stability and posttranscriptional regulation in general. All PCBPs contain three different KH domains and recognize poly(C)-sequences with high affinity and specificity. To reveal the molecular basis of poly(C)-sequence recognition, we have determined the crystal structure, at 1.6 A resolution, of PCBP2 KH3 domain in complex with a 7-nt DNA sequence (5'-AACCCTA-3') corresponding to one repeat of the C-rich strand of human telomeric DNA. The domain assumes a type-I KH fold in a betaalphaalphabetabetaalpha configuration. The protein-DNA interface could be studied in unprecedented detail and is made up of a series of direct and water-mediated hydrogen bonds between the protein and the DNA, revealing an especially dense network involving several structural water molecules for the last 2 nt in the core recognition sequence. Unlike published KH domain structures, the protein crystallizes without protein-protein contacts, yielding new insights into the dimerization properties of different KH domains. A nucleotide platform, an interesting feature found in some RNA molecules, was identified, evidently for the first time in DNA. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Du, Z.]] | [[Category: Du, Z.]] | ||
[[Category: Fenn, S.]] | [[Category: Fenn, S.]] | ||
| - | [[Category: James, T | + | [[Category: James, T L.]] |
| - | [[Category: Lee, J | + | [[Category: Lee, J K.]] |
| - | [[Category: Stroud, R | + | [[Category: Stroud, R M.]] |
[[Category: Tjhen, R.]] | [[Category: Tjhen, R.]] | ||
[[Category: CYT]] | [[Category: CYT]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:25:14 2008'' |
Revision as of 16:25, 21 February 2008
|
Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA
Overview
KH (hnRNP K homology) domains, consisting of approximately 70 amino acid residues, are present in a variety of nucleic-acid-binding proteins. Among these are poly(C)-binding proteins (PCBPs), which are important regulators of mRNA stability and posttranscriptional regulation in general. All PCBPs contain three different KH domains and recognize poly(C)-sequences with high affinity and specificity. To reveal the molecular basis of poly(C)-sequence recognition, we have determined the crystal structure, at 1.6 A resolution, of PCBP2 KH3 domain in complex with a 7-nt DNA sequence (5'-AACCCTA-3') corresponding to one repeat of the C-rich strand of human telomeric DNA. The domain assumes a type-I KH fold in a betaalphaalphabetabetaalpha configuration. The protein-DNA interface could be studied in unprecedented detail and is made up of a series of direct and water-mediated hydrogen bonds between the protein and the DNA, revealing an especially dense network involving several structural water molecules for the last 2 nt in the core recognition sequence. Unlike published KH domain structures, the protein crystallizes without protein-protein contacts, yielding new insights into the dimerization properties of different KH domains. A nucleotide platform, an interesting feature found in some RNA molecules, was identified, evidently for the first time in DNA.
About this Structure
2P2R is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution., Fenn S, Du Z, Lee JK, Tjhen R, Stroud RM, James TL, Nucleic Acids Res. 2007;35(8):2651-60. Epub 2007 Apr 10. PMID:17426136
Page seeded by OCA on Thu Feb 21 18:25:14 2008
Categories: Homo sapiens | Single protein | Du, Z. | Fenn, S. | James, T L. | Lee, J K. | Stroud, R M. | Tjhen, R. | CYT | Protein-dna complex
