This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


Sandbox Reserved 194

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(→Headline text)
Line 4: Line 4:
== '''Ribonuclease A Substrate Binding''' ==
== '''Ribonuclease A Substrate Binding''' ==
-
== Headline text ==
+
== '''Headline text''' ==
<Structure load='1RTA' size='450' frame='true' align='right' caption='Insert caption here' scene='Sandbox_Reserved_194/1rta_just_dt/1' />
<Structure load='1RTA' size='450' frame='true' align='right' caption='Insert caption here' scene='Sandbox_Reserved_194/1rta_just_dt/1' />
[[Image:1RTA_zoom.png|thumb|left|250px|Thymidylic acid tetramer complexed with ribonuclease A]]
[[Image:1RTA_zoom.png|thumb|left|250px|Thymidylic acid tetramer complexed with ribonuclease A]]

Revision as of 00:45, 31 March 2011

This Sandbox is Reserved from Feb 02, 2011, through Jul 31, 2011 for use by the Biochemistry II class at the Butler University at Indianapolis, IN USA taught by R. Jeremy Johnson. This reservation includes Sandbox Reserved 191 through Sandbox Reserved 200.
To get started:
  • Click the edit this page tab at the top. Save the page after each step, then edit it again.
  • Click the 3D button (when editing, above the wikitext box) to insert a 3D applet Jmol scene window.
  • show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page.
  • Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc.

More help: Help:Editing

Ribonuclease A Substrate Binding

Headline text

Insert caption here

Drag the structure with the mouse to rotate
Image:1RTA zoom.png
Thymidylic acid tetramer complexed with ribonuclease A

To determine the structural characteristics of RNA substrate binding to RNase A, X-ray crystallography was used to image inhibitory DNA tetramers bound to the enzyme. DNA lacks the 2′OH essential to RNA cleavage, making the complex more conducive to crystallography. In previous studies, has provided information into the specificity of the binding pocket subunits, B0, B1, B2 and B3.

Further binding pocket characterization was accomplished using the oglionucleotide . While binding of other nucleobases to the B2 and B3 sites is possible, the imaging of this elucidated the preference for adenosine bases. RNase A establishes in addition to hydrogen bonding between .

Image:1RCN zoom.png
ApTpApApG complexed with ribonuclease A
Retrieved from "http://52.214.119.220/wiki/index.php/Sandbox_Reserved_194"
Personal tools