2os8
From Proteopedia
(New page: 200px<br /><applet load="2os8" size="350" color="white" frame="true" align="right" spinBox="true" caption="2os8, resolution 3.270Å" /> '''Rigor-like structur...) |
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==Overview== | ==Overview== | ||
| - | Unlike processive cellular motors such as myosin V, whose structure has | + | Unlike processive cellular motors such as myosin V, whose structure has recently been determined in a "rigor-like" conformation, myosin II from contracting muscle filaments necessarily spends most of its time detached from actin. By using squid and sea scallop sources, however, we have now obtained similar rigor-like atomic structures for muscle myosin heads (S1). The significance of the hallmark closed actin-binding cleft in these crystal structures is supported here by actin/S1-binding studies. These structures reveal how different duty ratios, and hence cellular functions, of the myosin isoforms may be accounted for, in part, on the basis of detailed differences in interdomain contacts. Moreover, the rigor-like position of switch II turns out to be unique for myosin V. The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Rigor-like | + | Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor., Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C, Structure. 2007 May;15(5):553-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17502101 17502101] |
[[Category: Placopecten magellanicus]] | [[Category: Placopecten magellanicus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Brown, J | + | [[Category: Brown, J H.]] |
[[Category: Cohen, C.]] | [[Category: Cohen, C.]] | ||
[[Category: Gourinath, S.]] | [[Category: Gourinath, S.]] | ||
| Line 24: | Line 24: | ||
[[Category: rigor-like]] | [[Category: rigor-like]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:21:56 2008'' |
Revision as of 16:21, 21 February 2008
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Rigor-like structures of muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor
Overview
Unlike processive cellular motors such as myosin V, whose structure has recently been determined in a "rigor-like" conformation, myosin II from contracting muscle filaments necessarily spends most of its time detached from actin. By using squid and sea scallop sources, however, we have now obtained similar rigor-like atomic structures for muscle myosin heads (S1). The significance of the hallmark closed actin-binding cleft in these crystal structures is supported here by actin/S1-binding studies. These structures reveal how different duty ratios, and hence cellular functions, of the myosin isoforms may be accounted for, in part, on the basis of detailed differences in interdomain contacts. Moreover, the rigor-like position of switch II turns out to be unique for myosin V. The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states.
About this Structure
2OS8 is a Protein complex structure of sequences from Placopecten magellanicus with and as ligands. Full crystallographic information is available from OCA.
Reference
Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor., Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C, Structure. 2007 May;15(5):553-64. PMID:17502101
Page seeded by OCA on Thu Feb 21 18:21:56 2008
Categories: Placopecten magellanicus | Protein complex | Brown, J H. | Cohen, C. | Gourinath, S. | Yang, Y. | CA | MG | Mechanical elements | Motor | Myosin s1 | Rigor-like
