2oy6
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Unlike processive cellular motors such as myosin V, whose structure has | + | Unlike processive cellular motors such as myosin V, whose structure has recently been determined in a "rigor-like" conformation, myosin II from contracting muscle filaments necessarily spends most of its time detached from actin. By using squid and sea scallop sources, however, we have now obtained similar rigor-like atomic structures for muscle myosin heads (S1). The significance of the hallmark closed actin-binding cleft in these crystal structures is supported here by actin/S1-binding studies. These structures reveal how different duty ratios, and hence cellular functions, of the myosin isoforms may be accounted for, in part, on the basis of detailed differences in interdomain contacts. Moreover, the rigor-like position of switch II turns out to be unique for myosin V. The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | Rigor-like | + | Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor., Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C, Structure. 2007 May;15(5):553-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17502101 17502101] |
[[Category: Loligo plei]] | [[Category: Loligo plei]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Todarodes pacificus]] | [[Category: Todarodes pacificus]] | ||
| - | [[Category: Brown, J | + | [[Category: Brown, J H.]] |
[[Category: Cohen, C.]] | [[Category: Cohen, C.]] | ||
[[Category: Gourinath, S.]] | [[Category: Gourinath, S.]] | ||
| - | [[Category: Himmel, D | + | [[Category: Himmel, D M.]] |
[[Category: Kovacs, M.]] | [[Category: Kovacs, M.]] | ||
| - | [[Category: Neall-Hennessey, E | + | [[Category: Neall-Hennessey, E O.]] |
[[Category: Nyitray, L.]] | [[Category: Nyitray, L.]] | ||
[[Category: Reshetnikova, L.]] | [[Category: Reshetnikova, L.]] | ||
[[Category: Reutzel, R.]] | [[Category: Reutzel, R.]] | ||
| - | [[Category: Szent-Gyorgyi, A | + | [[Category: Szent-Gyorgyi, A G.]] |
[[Category: Yang, Y.]] | [[Category: Yang, Y.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| - | [[Category: mg | + | [[Category: mg adp]] |
[[Category: myosin ii s1]] | [[Category: myosin ii s1]] | ||
[[Category: post-rigor state]] | [[Category: post-rigor state]] | ||
[[Category: squid]] | [[Category: squid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:23:57 2008'' |
Revision as of 16:23, 21 February 2008
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Crystal structure of Squid Mg.ADP myosin S1
Overview
Unlike processive cellular motors such as myosin V, whose structure has recently been determined in a "rigor-like" conformation, myosin II from contracting muscle filaments necessarily spends most of its time detached from actin. By using squid and sea scallop sources, however, we have now obtained similar rigor-like atomic structures for muscle myosin heads (S1). The significance of the hallmark closed actin-binding cleft in these crystal structures is supported here by actin/S1-binding studies. These structures reveal how different duty ratios, and hence cellular functions, of the myosin isoforms may be accounted for, in part, on the basis of detailed differences in interdomain contacts. Moreover, the rigor-like position of switch II turns out to be unique for myosin V. The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states.
About this Structure
2OY6 is a Protein complex structure of sequences from Loligo plei and Todarodes pacificus with and as ligands. Full crystallographic information is available from OCA.
Reference
Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor., Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C, Structure. 2007 May;15(5):553-64. PMID:17502101
Page seeded by OCA on Thu Feb 21 18:23:57 2008
