Sandbox Reserved 311
From Proteopedia
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{{STRUCTURE_1e4g| PDB=1e4g | SCENE= }} | {{STRUCTURE_1e4g| PDB=1e4g | SCENE= }} | ||
=Introduction= | =Introduction= | ||
| - | FtsA is a peripheral membrane cell division protein which can bind ATP. It belongs to the actin family of proteins that includes actin, hsp 70, and sugar kinase. | + | FtsA is a peripheral membrane cell division protein which can bind ATP. It belongs to the actin family of proteins that includes actin, hsp 70, and sugar kinase.<ref name="Dewar">PMID: 1400183</ref> FtsA is an ATPase and is homologous to the ATPase domain of actin family of proteins. (4)FtsZ is another important cell division protein, <ref name="Dewar"> and is a structural homologue of eukaryotic tubulin (3). Polymerization of FtsZ is a crucial step in forming septal ring.<ref name="Dewar"> |
The bacterial cell cycle includes events such as initiation of chromosome replication, nucleoid segregation, and cell division. During the cell division, nine proteins will be localized in the cell center and will direct the assembly of the division septum. (2) | The bacterial cell cycle includes events such as initiation of chromosome replication, nucleoid segregation, and cell division. During the cell division, nine proteins will be localized in the cell center and will direct the assembly of the division septum. (2) | ||
| - | A collection of cell division proteins encoded by FtsA, FtsI, FtsK, FtsL, FtsN, FtsQ, FtsW, FtsZ and zipA are essential for bacterial cell division and function directly in the septation. (4) Assembly of ring proteins happens in a specific order starting with polymerization of the protein FtsZ into the Z ring, with the preceding protein dictating the recruitment of the next one. Localization of FtsA depends on the localization of FtsZ(4). FtsA will localize to the division site and bind to the FtsZ ring in order to connect the FtsZ molecules and membrane-anchored proteins or integral membrane protein of the septum | + | A collection of cell division proteins encoded by FtsA, FtsI, FtsK, FtsL, FtsN, FtsQ, FtsW, FtsZ and zipA are essential for bacterial cell division and function directly in the septation. (4) Assembly of ring proteins happens in a specific order starting with polymerization of the protein FtsZ into the Z ring, with the preceding protein dictating the recruitment of the next one. Localization of FtsA depends on the localization of FtsZ(4). FtsA will localize to the division site and bind to the FtsZ ring in order to connect the FtsZ molecules and membrane-anchored proteins or integral membrane protein of the septum. Then the rest of the proteins can assemble into the septal ring, which contracts as the septum grows. |
| - | A specific ratio of FtsA to FtsZ protein is required in cell division | + | A specific ratio of FtsA to FtsZ protein is required in cell division.<ref name="Dewar"> Overproductionof FtsZ can inhibit cell division and Z ring formation, thus preventing the localization of the FtsA.(4) |
==Structure == | ==Structure == | ||
The 3D structure of FtsA is similar to structure of the actin family of proteins. Not only are FtsA and actin resembles each other in structure, but they are also similar in their function since both of them are involved bacterial cytokinesis.FtsA contains two domains and each domain separates into two homologous subdomains, 1A and 2A. Both subdomains are composed of five-stranded β sheet surrounded by three helices. The other two smaller subdomains, 1C and 2B, vary amoung actin family and are different in structures. The <scene name='Sandbox_Reserved_311/Active_site/1'>nucleotide-binding site</scene> of FtsA is located in the interdomain cleft formedd by subdomains 1A, 2A and 2B(5). The adenosine is located in a hydrophobic pocket which is made of residues from subdomain 2A and 2B. The phosphate moiety of the nucleotide binds to two loops that connect the first and second strand of subdomain 1A and 2A. | The 3D structure of FtsA is similar to structure of the actin family of proteins. Not only are FtsA and actin resembles each other in structure, but they are also similar in their function since both of them are involved bacterial cytokinesis.FtsA contains two domains and each domain separates into two homologous subdomains, 1A and 2A. Both subdomains are composed of five-stranded β sheet surrounded by three helices. The other two smaller subdomains, 1C and 2B, vary amoung actin family and are different in structures. The <scene name='Sandbox_Reserved_311/Active_site/1'>nucleotide-binding site</scene> of FtsA is located in the interdomain cleft formedd by subdomains 1A, 2A and 2B(5). The adenosine is located in a hydrophobic pocket which is made of residues from subdomain 2A and 2B. The phosphate moiety of the nucleotide binds to two loops that connect the first and second strand of subdomain 1A and 2A. | ||
Revision as of 10:31, 4 April 2011
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| 1e4g, resolution 2.60Å () | |||||||||
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| Ligands: | , | ||||||||
| Non-Standard Residues: | |||||||||
| Gene: | FTSA (Thermotoga maritima) | ||||||||
| Related: | 1e4f | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Introduction
FtsA is a peripheral membrane cell division protein which can bind ATP. It belongs to the actin family of proteins that includes actin, hsp 70, and sugar kinase.[1] FtsA is an ATPase and is homologous to the ATPase domain of actin family of proteins. (4)FtsZ is another important cell division protein, [1]
