2h09

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(New page: 200px<br /><applet load="2h09" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h09, resolution 2.10&Aring;" /> '''Crystal structure of...)
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==Overview==
==Overview==
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The virulent phenotype of the pathogenic bacterium Corynebacterium, diphtheriae is conferred by diphtheria toxin, whose expression is an, adaptive response to low concentrations of iron. The expression of the, toxin gene (tox) is regulated by the repressor DtxR, which is activated by, transition metal ions. X-ray crystal structures of DtxR with and without, (apo-form) its coordinated transition metal ion have established the, general architecture of the repressor, identified the location of the, metal-binding sites, and revealed a metal-ion-triggered subunit-subunit, 'caliper-like' conformational change. Here we report the three-dimensional, crystal structure of the complex between a biologically active, Ni(II)-bound DtxR(C102D) mutant, in which a cysteine is replaced by an, aspartate at residue 102, and a 33-base-pair DNA segment containing the, toxin operator toxO. This structure shows that DNA interacts with two, dimeric repressor proteins bound to opposite sides of the tox operator. We, propose that a metal-ion-induced helix-to-coil structural transition in, the amino-terminal region of the protein is partly responsible for the, unique mode of repressor activation by transition metal ions.
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The virulent phenotype of the pathogenic bacterium Corynebacterium diphtheriae is conferred by diphtheria toxin, whose expression is an adaptive response to low concentrations of iron. The expression of the toxin gene (tox) is regulated by the repressor DtxR, which is activated by transition metal ions. X-ray crystal structures of DtxR with and without (apo-form) its coordinated transition metal ion have established the general architecture of the repressor, identified the location of the metal-binding sites, and revealed a metal-ion-triggered subunit-subunit 'caliper-like' conformational change. Here we report the three-dimensional crystal structure of the complex between a biologically active Ni(II)-bound DtxR(C102D) mutant, in which a cysteine is replaced by an aspartate at residue 102, and a 33-base-pair DNA segment containing the toxin operator toxO. This structure shows that DNA interacts with two dimeric repressor proteins bound to opposite sides of the tox operator. We propose that a metal-ion-induced helix-to-coil structural transition in the amino-terminal region of the protein is partly responsible for the unique mode of repressor activation by transition metal ions.
==About this Structure==
==About this Structure==
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Kumarevel, T.S.]]
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[[Category: Kumarevel, T S.]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
[[Category: Shinkai, A.]]
[[Category: Shinkai, A.]]
[[Category: Tanaka, T.]]
[[Category: Tanaka, T.]]
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[[Category: transcription regulator]]
[[Category: transcription regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:36:51 2008''

Revision as of 15:36, 21 February 2008

Image:2h09.jpg

2h09, resolution 2.10Å

Drag the structure with the mouse to rotate

Crystal structure of diphtheria toxin repressor like protein from E. coli

Overview

The virulent phenotype of the pathogenic bacterium Corynebacterium diphtheriae is conferred by diphtheria toxin, whose expression is an adaptive response to low concentrations of iron. The expression of the toxin gene (tox) is regulated by the repressor DtxR, which is activated by transition metal ions. X-ray crystal structures of DtxR with and without (apo-form) its coordinated transition metal ion have established the general architecture of the repressor, identified the location of the metal-binding sites, and revealed a metal-ion-triggered subunit-subunit 'caliper-like' conformational change. Here we report the three-dimensional crystal structure of the complex between a biologically active Ni(II)-bound DtxR(C102D) mutant, in which a cysteine is replaced by an aspartate at residue 102, and a 33-base-pair DNA segment containing the toxin operator toxO. This structure shows that DNA interacts with two dimeric repressor proteins bound to opposite sides of the tox operator. We propose that a metal-ion-induced helix-to-coil structural transition in the amino-terminal region of the protein is partly responsible for the unique mode of repressor activation by transition metal ions.

About this Structure

2H09 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the metal-ion-activated diphtheria toxin repressor/tox operator complex., White A, Ding X, vanderSpek JC, Murphy JR, Ringe D, Nature. 1998 Jul 30;394(6692):502-6. PMID:9697776

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