Non-polymerizable monomeric actin

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{{STRUCTURE_2hf4| PDB=2hf4 | SCENE=User:Thomas_E_Sladewski/Sandbox_1/2hf4_blue_helix2/1|CAPTION= Crystal structure of the VEGFR2 kinase domain in complex with a pyridyl-pyrimidine benzimidazole inhibitor, [[2HF4]] }}
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{{STRUCTURE_2hf4| PDB=2hf4 | SCENE=User:Thomas_E_Sladewski/Sandbox_1/2hf4_blue_helix2/1|CAPTION= Crystal structure of the AP-actin in complex with ATP, [[2HF4]] }}
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Non-polymerizable monomeric actin or AP-actin is an Sf9-expressed cytoplasmic actin harboring two point mutations that prevent the monomer from polymerizing into actin filaments. These mutations allow for the crystallization of actin without the use of specific toxins or actin-binding proteins that may influence the structure. The crystal structure of AP-actin has been solved for the ADP-bound form (PDB code 2HF4) and the ATP-bound form (PDB code 2HF3). These two structures are shown right as a morph between the two states.
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Non-polymerizable monomeric actin or AP-actin is an Sf9-expressed cytoplasmic actin harboring two point mutations that prevent the monomer from polymerizing into actin filaments. These mutations allow for the crystallization of actin without the use of specific toxins or actin-binding proteins that may influence the structure. The crystal structure of AP-actin has been solved for the ADP-bound form ([[2HF3]]) and the ATP-bound form ([[2HF4]]). These two structures are shown below as a morph between the two states.
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===Crystal structure of Monomeric Actin in its ATP-bound state===
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===Crystal structure of AP-actin in the ATP and ADP state===
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===Structural features of AP-actin===
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===Crystal structure of Monomeric Actin in its ATP-bound state===
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Revision as of 00:27, 12 April 2011

Template:STRUCTURE 2hf4


Non-polymerizable monomeric actin or AP-actin is an Sf9-expressed cytoplasmic actin harboring two point mutations that prevent the monomer from polymerizing into actin filaments. These mutations allow for the crystallization of actin without the use of specific toxins or actin-binding proteins that may influence the structure. The crystal structure of AP-actin has been solved for the ADP-bound form (2HF3) and the ATP-bound form (2HF4). These two structures are shown below as a morph between the two states.


Contents

Crystal structure of AP-actin in the ATP and ADP state

 
Drag the structure with the mouse to rotate
Morph of the lac repressor complexed with DNA showing the differences between non-specific binding (straight DNA) vs. specific recognition of the operator sequence (kinked DNA). Whether the binding kinks the DNA, or simply stabilizes a pre-existing kink, is unknown. Details.


Structural features of AP-actin

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Mechanism

About this Structure

This is where I will add my text. 2hf4 is a 1 chain structure of Actin with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

See Also

Reference

  • Rould MA, Wan Q, Joel PB, Lowey S, Trybus KM. Crystal structures of expressed non-polymerizable monomeric actin in the ADP and ATP states. J Biol Chem. 2006 Oct 20;281(42):31909-19. Epub 2006 Aug 18. PMID:16920713 doi:M601973200

Proteopedia Page Contributors and Editors (what is this?)

Thomas E Sladewski, Michal Harel, Alexander Berchansky

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