3nev

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[[Image:3nev.jpg|left|200px]]
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==Crystal structure of YagE, a prophage protein from E. coli K12 in complex with KDGal==
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<StructureSection load='3nev' size='340' side='right' caption='[[3nev]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nev]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NEV FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=RSH:3-DEOXY-D-LYXO-HEXONIC+ACID'>RSH</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2v8z|2v8z]], [[2v9d|2v9d]], [[3n2x|3n2x]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b0268, JW0261, yagE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nev RCSB], [http://www.ebi.ac.uk/pdbsum/3nev PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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YagE is a 33 kDa prophage protein encoded by CP4-6 prophage element in Escherichia coli K12 genome. Here, we report the structures of YagE complexes with pyruvate (PDB Id 3N2X) and KDGal (2-keto-3-deoxy galactonate) (PDB Id 3NEV) at 2.2A resolution. Pyruvate depletion assay in presence of glyceraldehyde shows that YagE catalyses the aldol condensation of pyruvate and glyceraldehyde. Our results indicate that the biochemical function of YagE is that of a 2-keto-3-deoxy gluconate (KDG) aldolase. Interestingly, E. coli K12 genome lacks an intrinsic KDG aldolase. Moreover, the over-expression of YagE increases cell viability in the presence of certain bactericidal antibiotics, indicating a putative biological role of YagE as a prophage encoded virulence factor enabling the survival of bacteria in the presence of certain antibiotics. The analysis implies a possible mechanism of antibiotic resistance conferred by the over-expression of prophage encoded YagE to E. coli. Proteins 2011, (c) 2011 Wiley-Liss, Inc.
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Identification of biochemical and putative biological role of a xenolog from Escherichia coli using structural analysis.,Bhaskar V, Kumar M, Manicka S, Tripathi S, Venkatraman A, Krishnaswamy S Proteins. 2011 Apr;79(4):1132-42. doi: 10.1002/prot.22949. Epub 2011 Feb, 3. PMID:21294156<ref>PMID:21294156</ref>
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The line below this paragraph, containing "STRUCTURE_3nev", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3nev| PDB=3nev | SCENE= }}
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===Crystal structure of YagE, a prophage protein from E. coli K12 in complex with KDGal===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21294156}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21294156 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21294156}}
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==About this Structure==
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[[3nev]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NEV OCA].
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==Reference==
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<ref group="xtra">PMID:21294156</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Bhaskar, V.]]
[[Category: Bhaskar, V.]]
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[[Category: Kumar, P M.]]
[[Category: Kumar, P M.]]
[[Category: Manicka, S.]]
[[Category: Manicka, S.]]
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[[Category: Aldolase]]
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[[Category: Lyase]]
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[[Category: Protein-ligand complex]]
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[[Category: Tim barrel]]

Revision as of 10:30, 28 May 2014

Crystal structure of YagE, a prophage protein from E. coli K12 in complex with KDGal

3nev, resolution 2.19Å

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