2pnf
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The gene product of fabG from Aquifex aeolicus has been heterologously | + | The gene product of fabG from Aquifex aeolicus has been heterologously expressed in Escherichia coli. Purification of the protein took place using anion-exchange and size-exclusion chromatography and the protein was then crystallized. Diffraction data were collected to a maximum resolution of 1.8 A and the initial phases were determined by molecular replacement. The A. aeolicus FabG protein is a putative beta-ketoacyl-acyl carrier protein reductase. Structure-function studies of this protein are being performed as part of a larger project investigating naturally occurring deviations from highly conserved residues within the short-chain oxidoreductase (SCOR) family. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Mao, Q.]] | [[Category: Mao, Q.]] | ||
| - | [[Category: Umland, T | + | [[Category: Umland, T C.]] |
[[Category: 1PE]] | [[Category: 1PE]] | ||
[[Category: MES]] | [[Category: MES]] | ||
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[[Category: short chain oxidoreductase]] | [[Category: short chain oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:31:17 2008'' |
Revision as of 16:31, 21 February 2008
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Structure of Aquifex Aeolicus FabG 3-oxoacyl-(acyl-carrier protein) reductase
Overview
The gene product of fabG from Aquifex aeolicus has been heterologously expressed in Escherichia coli. Purification of the protein took place using anion-exchange and size-exclusion chromatography and the protein was then crystallized. Diffraction data were collected to a maximum resolution of 1.8 A and the initial phases were determined by molecular replacement. The A. aeolicus FabG protein is a putative beta-ketoacyl-acyl carrier protein reductase. Structure-function studies of this protein are being performed as part of a larger project investigating naturally occurring deviations from highly conserved residues within the short-chain oxidoreductase (SCOR) family.
About this Structure
2PNF is a Single protein structure of sequence from Aquifex aeolicus with and as ligands. Active as [acyl-carrier-protein_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number 1.1.1.100 Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray diffraction analysis of the beta-ketoacyl-acyl carrier protein reductase FabG from Aquifex aeolicus VF5., Mao Q, Duax WL, Umland TC, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Feb 1;63(Pt, 2):106-9. Epub 2007 Jan 17. PMID:17277451 [[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]]
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