1zoy
From Proteopedia
(New page: 200px<br /><applet load="1zoy" size="350" color="white" frame="true" align="right" spinBox="true" caption="1zoy, resolution 2.4Å" /> '''Crystal Structure of ...) |
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==Overview== | ==Overview== | ||
| - | The mitochondrial respiratory Complex II or succinate:ubiquinone | + | The mitochondrial respiratory Complex II or succinate:ubiquinone oxidoreductase (SQR) is an integral membrane protein complex in both the tricarboxylic acid cycle and aerobic respiration. Here we report the first crystal structure of Complex II from porcine heart at 2.4 A resolution and its complex structure with inhibitors 3-nitropropionate and 2-thenoyltrifluoroacetone (TTFA) at 3.5 A resolution. Complex II is comprised of two hydrophilic proteins, flavoprotein (Fp) and iron-sulfur protein (Ip), and two transmembrane proteins (CybL and CybS), as well as prosthetic groups required for electron transfer from succinate to ubiquinone. The structure correlates the protein environments around prosthetic groups with their unique midpoint redox potentials. Two ubiquinone binding sites are discussed and elucidated by TTFA binding. The Complex II structure provides a bona fide model for study of the mitochondrial respiratory system and human mitochondrial diseases related to mutations in this complex. |
==About this Structure== | ==About this Structure== | ||
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[[Category: ubiquinone oxidoreductase]] | [[Category: ubiquinone oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:17:42 2008'' |
Revision as of 14:17, 21 February 2008
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Crystal Structure of Mitochondrial Respiratory Complex II from porcine heart at 2.4 Angstroms
Overview
The mitochondrial respiratory Complex II or succinate:ubiquinone oxidoreductase (SQR) is an integral membrane protein complex in both the tricarboxylic acid cycle and aerobic respiration. Here we report the first crystal structure of Complex II from porcine heart at 2.4 A resolution and its complex structure with inhibitors 3-nitropropionate and 2-thenoyltrifluoroacetone (TTFA) at 3.5 A resolution. Complex II is comprised of two hydrophilic proteins, flavoprotein (Fp) and iron-sulfur protein (Ip), and two transmembrane proteins (CybL and CybS), as well as prosthetic groups required for electron transfer from succinate to ubiquinone. The structure correlates the protein environments around prosthetic groups with their unique midpoint redox potentials. Two ubiquinone binding sites are discussed and elucidated by TTFA binding. The Complex II structure provides a bona fide model for study of the mitochondrial respiratory system and human mitochondrial diseases related to mutations in this complex.
About this Structure
1ZOY is a Protein complex structure of sequences from Sus scrofa with , , , , , and as ligands. Active as Succinate dehydrogenase (ubiquinone), with EC number 1.3.5.1 Full crystallographic information is available from OCA.
Reference
Crystal structure of mitochondrial respiratory membrane protein complex II., Sun F, Huo X, Zhai Y, Wang A, Xu J, Su D, Bartlam M, Rao Z, Cell. 2005 Jul 1;121(7):1043-57. PMID:15989954
Page seeded by OCA on Thu Feb 21 16:17:42 2008
Categories: Protein complex | Succinate dehydrogenase (ubiquinone) | Sus scrofa | Bartlam, M. | Huo, X. | Rao, Z. | Su, D. | Sun, F. | Wang, A. | Xu, J. | Zhai, Y. | EPH | F3S | FAD | FES | HEM | SF4 | UQ1 | Membrane protein structure | Mitochondrial respiratory complex ii | Succinate | Ubiquinone oxidoreductase
