211d
From Proteopedia
(New page: 200px<br /><applet load="211d" size="350" color="white" frame="true" align="right" spinBox="true" caption="211d, resolution 1.600Å" /> '''THE CRYSTAL AND MOL...) |
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==Overview== | ==Overview== | ||
| - | The three-dimensional structure of d[CGTA'CG], where A' = [2-NH2-A], was | + | The three-dimensional structure of d[CGTA'CG], where A' = [2-NH2-A], was determined to atomic (1.35 A) resolution by single isomorphous replacement. The d[CGTA'CG] hexamer crystallizes in space group P3221, and is not isomorphous with other DNA hexanucleotides. Despite completely different crystal packing, the essential characteristics of the Z-DNA conformation are maintained. The structure was determined by single isomorphous replacement using a triammine platinum fragment. Thus, this study also demonstrates, for the first time, the feasibility of the use of this reagent for the direct phasing of DNA crystal structures. |
==About this Structure== | ==About this Structure== | ||
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Crystal and molecular structure of a new Z-DNA crystal form: d[CGT(2-NH2-A)CG] and its platinated derivative., Parkinson GN, Arvanitis GM, Lessinger L, Ginell SL, Jones R, Gaffney B, Berman HM, Biochemistry. 1995 Nov 28;34(47):15487-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7492550 7492550] | Crystal and molecular structure of a new Z-DNA crystal form: d[CGT(2-NH2-A)CG] and its platinated derivative., Parkinson GN, Arvanitis GM, Lessinger L, Ginell SL, Jones R, Gaffney B, Berman HM, Biochemistry. 1995 Nov 28;34(47):15487-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7492550 7492550] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Arvantis, G | + | [[Category: Arvantis, G M.]] |
| - | [[Category: Berman, H | + | [[Category: Berman, H M.]] |
[[Category: Gaffney, B.]] | [[Category: Gaffney, B.]] | ||
| - | [[Category: Ginell, S | + | [[Category: Ginell, S L.]] |
[[Category: Jones, R.]] | [[Category: Jones, R.]] | ||
[[Category: Lessinger, L.]] | [[Category: Lessinger, L.]] | ||
| - | [[Category: Parkinson, G | + | [[Category: Parkinson, G N.]] |
[[Category: PTN]] | [[Category: PTN]] | ||
[[Category: SPM]] | [[Category: SPM]] | ||
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[[Category: z-dna]] | [[Category: z-dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:58 2008'' |
Revision as of 14:20, 21 February 2008
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THE CRYSTAL AND MOLECULAR STRUCTURE OF A NEW Z-DNA CRYSTAL FORM: D[CGT(2-NH2-A) CG] AND ITS PLATINATED DERIVATIVE
Overview
The three-dimensional structure of d[CGTA'CG], where A' = [2-NH2-A], was determined to atomic (1.35 A) resolution by single isomorphous replacement. The d[CGTA'CG] hexamer crystallizes in space group P3221, and is not isomorphous with other DNA hexanucleotides. Despite completely different crystal packing, the essential characteristics of the Z-DNA conformation are maintained. The structure was determined by single isomorphous replacement using a triammine platinum fragment. Thus, this study also demonstrates, for the first time, the feasibility of the use of this reagent for the direct phasing of DNA crystal structures.
About this Structure
211D is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal and molecular structure of a new Z-DNA crystal form: d[CGT(2-NH2-A)CG] and its platinated derivative., Parkinson GN, Arvanitis GM, Lessinger L, Ginell SL, Jones R, Gaffney B, Berman HM, Biochemistry. 1995 Nov 28;34(47):15487-95. PMID:7492550
Page seeded by OCA on Thu Feb 21 16:20:58 2008
Categories: Protein complex | Arvantis, G M. | Berman, H M. | Gaffney, B. | Ginell, S L. | Jones, R. | Lessinger, L. | Parkinson, G N. | PTN | SPM | Double helix | Modified | Z-dna
