225d
From Proteopedia
(New page: 200px<br /><applet load="225d" size="350" color="white" frame="true" align="right" spinBox="true" caption="225d" /> '''A TETRAMERIC DNA STRUCTURE WITH PROTONATED C...) |
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==Overview== | ==Overview== | ||
| - | Oligomers containing tracts of cytidine form hemiprotonated base pairs at | + | Oligomers containing tracts of cytidine form hemiprotonated base pairs at acid pH and have been considered to be double-stranded. We have solved the structure of the DNA oligomer 5'-d(TCCCCC) at acid pH and find that it is a four-stranded complex in which two base-paired parallel-stranded duplexes are intimately associated, with their base pairs fully intercalated. The relative orientation of the duplexes is antiparallel, so that each base pair is face-to-face with its neighbours. The NMR spectrum displays only six spin systems, showing that the structure is highly symmetrical on the NMR timescale; the four strands are equivalent. A model derived by energy minimization and constrained molecular dynamics shows excellent compatibility with the observed nuclear Overhauser effects (NOEs) particularly for the very unusual inter-residue sugar-sugar NOEs H1'-H1', H1'-H2" and H1'-H4'. These NOEs are probably diagnostic for such tetrameric structures. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Gehring, K.]] | [[Category: Gehring, K.]] | ||
[[Category: Gueron, M.]] | [[Category: Gueron, M.]] | ||
| - | [[Category: Leroy, J | + | [[Category: Leroy, J L.]] |
[[Category: dna]] | [[Category: dna]] | ||
[[Category: intercalated cytosine tetrad]] | [[Category: intercalated cytosine tetrad]] | ||
[[Category: nmr]] | [[Category: nmr]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:21:14 2008'' |
Revision as of 14:21, 21 February 2008
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A TETRAMERIC DNA STRUCTURE WITH PROTONATED CYTOSINE:CYTOSINE BASE PAIRS
Overview
Oligomers containing tracts of cytidine form hemiprotonated base pairs at acid pH and have been considered to be double-stranded. We have solved the structure of the DNA oligomer 5'-d(TCCCCC) at acid pH and find that it is a four-stranded complex in which two base-paired parallel-stranded duplexes are intimately associated, with their base pairs fully intercalated. The relative orientation of the duplexes is antiparallel, so that each base pair is face-to-face with its neighbours. The NMR spectrum displays only six spin systems, showing that the structure is highly symmetrical on the NMR timescale; the four strands are equivalent. A model derived by energy minimization and constrained molecular dynamics shows excellent compatibility with the observed nuclear Overhauser effects (NOEs) particularly for the very unusual inter-residue sugar-sugar NOEs H1'-H1', H1'-H2" and H1'-H4'. These NOEs are probably diagnostic for such tetrameric structures.
About this Structure
225D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A tetrameric DNA structure with protonated cytosine.cytosine base pairs., Gehring K, Leroy JL, Gueron M, Nature. 1993 Jun 10;363(6429):561-5. PMID:8389423
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