229d
From Proteopedia
(New page: 200px<br /><applet load="229d" size="350" color="white" frame="true" align="right" spinBox="true" caption="229d" /> '''DNA ANALOG OF YEAST TRANSFER RNA PHE ANTICOD...) |
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==Overview== | ==Overview== | ||
| - | Design of biologically active DNA analogues of the yeast tRNA(Phe) | + | Design of biologically active DNA analogues of the yeast tRNA(Phe) anticodon domain, tDNAPheAC, required the introduction of a d(m5C)-dependent, Mg(2+)-induced structural transition and the d(m1G) disruption of an intra-loop dC.dG base pair. The modifications were introduced at residues corresponding to m5C-40 and wybutosine-37 in tRNA(Phe). Modified tDNAPheAC inhibited translation by 50% at a tDNAPheAC:ribosome ratio of 8:1. The molecule's structure has been determined by NMR spectroscopy and restrained molecular dynamics with an overall r.m.s.d. of 2.8 A and 1.7 A in the stem, and is similar to the tRNA(Phe) anticodon domain in conformation and dimensions. The tDNAPheAC structure may provide a guide for the design of translation inhibitors as potential therapeutic agents. |
==About this Structure== | ==About this Structure== | ||
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Design, biological activity and NMR-solution structure of a DNA analogue of yeast tRNA(Phe) anticodon domain., Basti MM, Stuart JW, Lam AT, Guenther R, Agris PF, Nat Struct Biol. 1996 Jan;3(1):38-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8548453 8548453] | Design, biological activity and NMR-solution structure of a DNA analogue of yeast tRNA(Phe) anticodon domain., Basti MM, Stuart JW, Lam AT, Guenther R, Agris PF, Nat Struct Biol. 1996 Jan;3(1):38-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8548453 8548453] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Agris, P | + | [[Category: Agris, P F.]] |
| - | [[Category: Basti, M | + | [[Category: Basti, M M.]] |
[[Category: Guenther, R.]] | [[Category: Guenther, R.]] | ||
| - | [[Category: Lam, A | + | [[Category: Lam, A T.]] |
| - | [[Category: Stuart, J | + | [[Category: Stuart, J W.]] |
[[Category: anticodon]] | [[Category: anticodon]] | ||
[[Category: dna]] | [[Category: dna]] | ||
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[[Category: transfer rna]] | [[Category: transfer rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:21:18 2008'' |
Revision as of 14:21, 21 February 2008
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DNA ANALOG OF YEAST TRANSFER RNA PHE ANTICODON DOMAIN WITH MODIFIED BASES 5-METHYL CYTOSINE AND 1-METHYL GUANINE
Overview
Design of biologically active DNA analogues of the yeast tRNA(Phe) anticodon domain, tDNAPheAC, required the introduction of a d(m5C)-dependent, Mg(2+)-induced structural transition and the d(m1G) disruption of an intra-loop dC.dG base pair. The modifications were introduced at residues corresponding to m5C-40 and wybutosine-37 in tRNA(Phe). Modified tDNAPheAC inhibited translation by 50% at a tDNAPheAC:ribosome ratio of 8:1. The molecule's structure has been determined by NMR spectroscopy and restrained molecular dynamics with an overall r.m.s.d. of 2.8 A and 1.7 A in the stem, and is similar to the tRNA(Phe) anticodon domain in conformation and dimensions. The tDNAPheAC structure may provide a guide for the design of translation inhibitors as potential therapeutic agents.
About this Structure
229D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Design, biological activity and NMR-solution structure of a DNA analogue of yeast tRNA(Phe) anticodon domain., Basti MM, Stuart JW, Lam AT, Guenther R, Agris PF, Nat Struct Biol. 1996 Jan;3(1):38-44. PMID:8548453
Page seeded by OCA on Thu Feb 21 16:21:18 2008
Categories: Protein complex | Agris, P F. | Basti, M M. | Guenther, R. | Lam, A T. | Stuart, J W. | Anticodon | Dna | Hairpin loop | Transfer rna
